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Gagnep., as well as and nine previously reported compounds (<jats:bold>2<\/jats:bold>\u2013<jats:bold>7<\/jats:bold> and <jats:bold>10<\/jats:bold>\u2013<jats:bold>12<\/jats:bold>). The structures and absolute configurations of <jats:bold>1<\/jats:bold>, <jats:bold>8<\/jats:bold>, and <jats:bold>9<\/jats:bold> were elucidated through comprehensive spectroscopic analyses, including two\u2010dimensional nuclear magnetic resonance, high\u2010resolution mass spectrometry, and infrared spectroscopy, as well as electronic circular dichroism calculations. Structurally, compound <jats:bold>8<\/jats:bold> is a rare ecdysteroid featuring a hexadienone unit, and <jats:bold>9<\/jats:bold> is an unprecedented ecdysteroid characterized by an oxygen bridge between C\u20102 and C\u20109. Additionally, compound <jats:bold>7<\/jats:bold> exhibited moderate inhibitory activity against nitric oxide production in lipopolysaccharide\u2010induced RAW 264.7 cells, with an IC<jats:sub>50<\/jats:sub> value of 35.78 \u00b1 0.37\u00a0\u00b5M, compared to the positive control, N<jats:sup>G<\/jats:sup>\u2010monomethyl\u2010L\u2010arginine, which had an IC<jats:sub>50<\/jats:sub> value of 40.28 \u00b1 0.48\u00a0\u00b5M.<\/jats:p>","DOI":"10.1002\/cbdv.202501502","type":"journal-article","created":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T20:58:13Z","timestamp":1749848293000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Clerodane Diterpenoids and Ecdysteroids From the Fruits of <i>Tinospora sagittata<\/i> (Oliv.) Gagnep."],"prefix":"10.1002","author":[{"given":"Rong\u2010Qing","family":"Yuan","sequence":"first","affiliation":[{"name":"Key Laboratory of Ethnic Medicine Resource Chemistry, Ministry of Education Yunnan Minzu University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai\u2010Yin","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences &amp; Yunnan Key Laboratory of Pharmacology for Natural Products Kunming Medical University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhou","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ethnic Medicine Resource Chemistry, Ministry of Education Yunnan Minzu University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Jia","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ethnic Medicine Resource Chemistry, Ministry of Education Yunnan Minzu University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010Xuan","family":"Ma","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ethnic Medicine Resource Chemistry, Ministry of Education Yunnan Minzu University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Run\u2010Cheng","family":"Yan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ethnic Medicine Resource Chemistry, Ministry of Education Yunnan Minzu University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi\u2010Huan","family":"Yin","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences &amp; Yunnan Key Laboratory of Pharmacology for Natural Products Kunming Medical University  Kunming China"},{"name":"College of Modern Biomedical Industry Kunming Medical University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Chao","family":"Tu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ethnic Medicine Resource Chemistry, Ministry of Education Yunnan Minzu University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Hu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ethnic Medicine Resource Chemistry, Ministry of Education Yunnan Minzu University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin\u2010Fen","family":"Ding","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences &amp; Yunnan Key Laboratory of Pharmacology for Natural Products Kunming Medical University  Kunming China"},{"name":"College of Modern Biomedical Industry Kunming Medical University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2526-1269","authenticated-orcid":false,"given":"Xing\u2010De","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Ethnic Medicine Resource Chemistry, Ministry of Education Yunnan Minzu University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,13]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Flora Reipublicae Popularis Sinicae","year":"2004"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","DOI":"10.1002\/cbdv.202302037","article-title":"A Review on Traditional Uses, Phytochemistry, Pharmacology and Clinical Application of Tinospora sinensis (Lour.) 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