{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T06:59:53Z","timestamp":1775890793746,"version":"3.50.1"},"reference-count":66,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T00:00:00Z","timestamp":1770249600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Type II diabetes mellites (TIIDM) characterized by hyperglycemia, insulin resistance, insensitivity, and pancreatic \u03b2\u2010cell atrophy has gained concern due to high rise in such cases globally. This study highlighted the therapeutic potency of a novel polyherbal formulation (PHF) of\n                    <jats:italic>Phyllanthus urinaria<\/jats:italic>\n                    and\n                    <jats:italic>Adhatoda vasica<\/jats:italic>\n                    Nees mice by in vitro, in vivo, and in silico analysis in high\u2010fat diet (HFD)\u2013streptozotocin (STZ)\u2010induced Swiss albino. The findings showed significant inhibition of \u03b1\u2010amylase and \u03b1\u2010glucosidase activity of the PHF along with decreased blood glucose level, increased glycogen and serum insulin level, elevated mRNA expression of GIPR and GLP1R, GLUT2, GLUT4, INSR, INS1, INS2, TCF7L2, and Pdx1 in both low and high dose of PHF\u2010treated mice as compared to HFD\u2013STZ\u2010induced diabetic mice. Western blot results also demonstrated augmented insulin protein level in both PHF\u2010treated groups. Okanin and vomicine, identified from LCMS analysis as potent antidiabetic bioactive compounds bind to dipeptidyl peptidase 4 (DPP4) with a binding energy of \u22128.04 and \u22127.81\u00a0kcal\/mol, respectively, as compared to standard drug metformin (\u22125.33\u00a0kcal\/mol). Inhibition of DDP\u20104 by bioactives of PHF aids in enhanced secretion of incretion hormones leading to insulin secretion thereby established itself as a complementary and alternative therapeutics in the management of diet\u2010induced TIIDM.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202501544","type":"journal-article","created":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T21:26:39Z","timestamp":1770326799000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["DPP4 and Insulin Protein: Drug Targets for a Formulated Polyherbal Combination of\n                    <i>Phyllanthus urinaria<\/i>\n                    L. and\n                    <i>Adhatoda vasica<\/i>\n                    Nees Against HFD\u2013STZ\u2010Induced TIIDM in Swiss Albino Mice"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6897-8572","authenticated-orcid":false,"given":"Pritimoni","family":"Das","sequence":"first","affiliation":[{"name":"Department of Zoology Mangaldai College  Mangaldai India"},{"name":"Department of Zoology Gauhati University  Guwahati India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6942-4213","authenticated-orcid":false,"given":"Manas","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Zoology Gauhati University  Guwahati India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pranjan","family":"Barman","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Gauhati University  Guwahati India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naba Kumar","family":"Hazarika","sequence":"additional","affiliation":[{"name":"Department of Rasa Shasra and Bhaishajya Kalpana Govt. 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