{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T12:47:08Z","timestamp":1775998028294,"version":"3.50.1"},"reference-count":54,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:00:00Z","timestamp":1773360000000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Carbonic anhydrase IX (CA IX) is overexpressed in many solid tumors, contributing to cancer cell proliferation, survival, invasion, and metastasis. Sulfonamide\u2010based compounds have emerged as potential anticancer agents by inhibiting this enzyme. In this study, we investigated the anticancer potential of a previously synthesized sulfonamide derivative, MMH\u2010I, focusing on its CA IX\u2010targeted activity and therapeutic efficacy\u00a0in both\u00a0in vitro\u00a0and\u00a0in vivo\u00a0models of breast cancer. Cytotoxicity was assessed using MTT assays in 4T1 breast cancer cells, while apoptosis was evaluated by acridine orange\/ethidium bromide staining and Annexin V detection. In vivo studies analyzed tumor tissues for CA IX expression, as well as Vimentin, E\u2010Cadherin, and Caspase\u20103 levels. H&amp;E staining and plasma metabolomic analysis were performed to assess tissue morphology and metabolic alterations. The compound significantly reduced tumor volume, induced apoptosis, and altered cancer\u2010related gene expression and metabolic profiles. Overall, this study provides a detailed in vivo and metabolic evaluation of MMH\u2010I in breast cancer, highlighting its potential as a CA IX\u2013targeted therapeutic candidate and supporting further investigation of sulfonamide\u2010based combination strategies against hypoxic tumors.<\/jats:p>","DOI":"10.1002\/cbdv.202501618","type":"journal-article","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T09:31:51Z","timestamp":1773394311000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["CA IX Inhibition by a Sulfonamide Compound: A Therapeutic Approach Against Breast Cancer"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4992-0281","authenticated-orcid":false,"given":"S\u00fckr\u00fc","family":"Akmese","sequence":"first","affiliation":[{"name":"Department of Medicinal Biochemistry, Medical Faculty Harran University  Sanl\u0131urfa 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