{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T13:08:56Z","timestamp":1773493736038,"version":"3.50.1"},"reference-count":54,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T00:00:00Z","timestamp":1757548800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T00:00:00Z","timestamp":1757548800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The current research work aims to characterize and evaluate cardioprotective and anti\u2010lipidemic effects of Ajwa date extracts on testosterone enanthate (TE)\u2010induced polycystic ovary syndrome (PCOS) in female albino mice. The phyto\u2010profilling of date extracts was analyzed using high\u2010performance liquid chromatography (HPLC) following standard methods. The PCOS was experimentally induced in the mice by intramuscular infusion of TE for 35 days. Ajwa date seed extract (ADSE) and Ajwa date whole extract (ADWE) were administered for 14 days at two different doses: 1 and 2\u00a0g\/kg\/BW. Serum creatine kinase myocardial band (CK\u2010MB) and lipid profiles were measured. The results showed that PCOS group exhibited elevated activities of serum CK\u2010MB and altered lipid profiles. Histological analysis of G2 (PCOS group) showed altered architecture of cardiac tissues. The molecular docking was also performed to support the results of in vivo studies. The HPLC characterization revealed that both extracts contained various polyphenols, quercetin, gallic acid, catechin (was found maximum in ADWE), and rutin (was found maximum in ADSE). The biochemical results revealed a significant decrease (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.001) in CK\u2010MB levels and lipid profile, including cholesterol, triglycerides, and low\u2010density lipids, and an increase in high\u2010density lipids after the administration of both extracts. Ajwa treatment normalized the cardiac tissue structure by reducing fibrosis and hemorrhagic zones and bettering fiber muscle organization. Notably, the higher doses of G5 and G6 (2\u00a0g\/kg\/BW) for both ADSE and ADWE provided superior myocardial protection and morphological preservation compared to the 1\u00a0g\/kg\/BW dose. Computation confirmation revealed that the detected bioactive compounds of extracts exhibited inhibitory potential against angiotensin receptor 1 and cyclooxygenase 2, which causes the cardiotoxicity. Especially, the rutin showed maximum inhibitory potential with binding energies of \u22129.2 and \u22129.8\u00a0kcal\/mol against angiotensin receptor 1 and cyclooxygenase 2, respectively. It is concluded that both ADSE and ADWE contained polyphenolic compounds that are involved in preventing the PCOS\u2010induced cardiotoxicity.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202501625","type":"journal-article","created":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T20:30:31Z","timestamp":1757622631000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of Date (\n                    <i>Phoenix dactylifera<\/i>\n                    ) Extracts in Alleviating PCOS\u2010Induced Myocardial Toxicity in Female Albino Mice (\n                    <i>Mus musculus<\/i>\n                    ): In Silico and In Vivo Approaches"],"prefix":"10.1002","volume":"22","author":[{"given":"Maryam","family":"Sana","sequence":"first","affiliation":[{"name":"Department of Zoology University of Sargodha  Sargodha Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Arshad","sequence":"additional","affiliation":[{"name":"Department of Zoology University of Sargodha  Sargodha Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farzana","family":"Siddique","sequence":"additional","affiliation":[{"name":"Institute of Food Science and Nutrition University of Sargodha  Sargodha Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uzma","family":"Altaf","sequence":"additional","affiliation":[{"name":"Govt. Queen Mary Graduate College Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hafiz Muhammad","family":"Irfan","sequence":"additional","affiliation":[{"name":"College of Pharmacy University of Sargodha  Sargodha Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rehab","family":"Rehman","sequence":"additional","affiliation":[{"name":"Department of Zoology University of Sargodha  Sargodha Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Tahir","family":"Akhtar","sequence":"additional","affiliation":[{"name":"Institute of Chemistry University of Sargodha  Sargodha Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Imam","family":"Bux","sequence":"additional","affiliation":[{"name":"Department of Agriculture Technology, Faculty of Agriculture Universiti Putra Malaysia  Serdang Selangor Malaysia"},{"name":"Department of Agriculture Education Extension, Faculty of Agricultural Social Sciences Sindh Agriculture University  Hyderabad Sindh Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0112-7594","authenticated-orcid":false,"given":"Rahman","family":"Qadir","sequence":"additional","affiliation":[{"name":"Department of Food Technology, Faculty of Food Science and Technology Universiti Putra Malaysia  Serdang Selangor Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.endmts.2025.100228"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejendo\/lvad077"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2015.05.005"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.2174\/0115701611269146230920073301"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2024.1410914"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmaa029"},{"issue":"5","key":"e_1_2_8_8_1","article-title":"Cardio\u2010Metabolic Disease and Polycystic Ovarian Syndrome (PCOS): A Narrative Review","volume":"14","author":"Sangaraju S. 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