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The conventional method to overcome inflammation is use of synthetic drugs, that is, diclofenac, ibuprofen, naproxen, and aspirin. These synthetic drugs not only reduce pain at that time but also cause drastic effects on the vital organs like the kidney, liver, and digestive tract. There is a serious need to develop herbal remedies because the world is returning into natural, safe, and organic medicines. The present study was proposed to attain sustainable development goal 3 (good health and well\u2010being). Plant\u2010mediated nanoparticles (NPs) offer regulated drug release, being sustainable, target\u2010specific, small sized, and eco\u2010friendly. Current study evaluated the in vivo and in vitro anti\u2010inflammatory, antibacterial, and antioxidant properties of aqueous leaf extract of Solanum nigrum L. and Solanum americanum M. Biofabricated zinc oxide NPs and formulation of wound healing herbal bandage as well as in silico studies, were also performed. Their efficacy was also explored through in vitro anti\u2010inflammatory activity (hemolysis assays and protein denaturation inhibition assay) and in vivo anti\u2010inflammatory activity (wound healing in rabbits), including histopathological analysis. In vitro results depicted that\n                    <jats:italic>S. nigrum<\/jats:italic>\n                    aqueous leaf extract exhibited lowest % age of hemolysis (0.1\u00a0\u00b1\u00a00.0005) at 50\u00a0\u00b5L concentration, whereas\n                    <jats:italic>S. americanum<\/jats:italic>\n                    mediated ZnO NPs displayed highest % of hemolysis (0.08\u00a0\u00b1\u00a00.0055) at 100\u00a0\u00b5L concentration. In vivo results indicated that bandages coated with S. nigrum aqueous extract and S. americanum significantly improved wound healing. ZnO nanocoated bandage exhibited the paramount anti\u2010inflammatory properties, suggesting it could be a viable alternative to conventional allopathic drugs. In silico results identified 112 inflammation\u2010associated gene targets, whereas tumor necrosis factor (TNF), IL6, and AKT1 were identified as top hub genes. Gas chromatography\u2013mass spectrometry (GC\u2013MS) and network pharmacology analysis identified key phytochemicals like kaempferol and quercetin. These phytochemicals target TNF, IL6, and AKT1 for drug repurposing, particularly to manage inflammation.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202501742","type":"journal-article","created":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:51:55Z","timestamp":1761155515000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Phytofabrication of Zinc Oxide Nanoparticle for Wound Management: Insights From In Vitro, In Vivo, and In Silico Studies"],"prefix":"10.1002","volume":"22","author":[{"given":"Munaza","family":"Khan","sequence":"first","affiliation":[{"name":"Department of Botany Lahore College for Women University  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zainab","family":"Maqbool","sequence":"additional","affiliation":[{"name":"Department of Botany Lahore College for Women University  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1525-3671","authenticated-orcid":false,"given":"Arusa","family":"Aftab","sequence":"additional","affiliation":[{"name":"Department of Botany Lahore College for Women University  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zubaida","family":"Yousaf","sequence":"additional","affiliation":[{"name":"Department of Botany Lahore College for Women University  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Awais","family":"Khalid","sequence":"additional","affiliation":[{"name":"Department of Physics, College of Science and Humanities Prince Sattam Bin Abdulaziz University  Al\u2010Kharj Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aneela Zameer","family":"Durrani","sequence":"additional","affiliation":[{"name":"University of Veterinary and Animal Sciences  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.23208"},{"issue":"4","key":"e_1_2_10_3_1","first-page":"1641","article-title":"A Review On Medicinal Plants Used as Anti\u2010Inflammatory Agents","volume":"8","author":"Virshette S.","year":"2019","journal-title":"Journal of Pharmacognosy and Phytochemistry"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2024.118137"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3828"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.3336"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25163726"},{"issue":"3","key":"e_1_2_10_8_1","first-page":"569","article-title":"Antifungal Activity of Vegetative Methanolic Extracts of Nigella sativa L. 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