{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T13:10:22Z","timestamp":1773493822356,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T00:00:00Z","timestamp":1756080000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T00:00:00Z","timestamp":1756080000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The regulation of vascular tone plays a fundamental role in blood pressure homeostasis and still represents a significant challenge in clinical practice. Boldine, a naturally occurring alkaloid from\n                    <jats:italic>Peumus boldus<\/jats:italic>\n                    , has emerged as a compound of interest due to its therapeutic potential. This study explored boldine's vasorelaxant effects on aortas of normotensive (NTR) and spontaneously hypertensive (SHR) rats.\n                    <jats:italic>N<\/jats:italic>\n                    \u03c9\u2010Nitro\u2010\n                    <jats:sc>l<\/jats:sc>\n                    \u2010arginine methyl ester\n                    <jats:sc>(l<\/jats:sc>\n                    \u2010NAME) (a nitric oxide synthase inhibitor), ODQ (a soluble guanylate cyclase inhibitor), and tetraethylammonium (TEA) (a nonselective K\u207a channel blocker) exerted a modest inhibitory effect, causing a rightward shift in the boldine\u2010induced relaxation curves without significantly affecting the maximal relaxation. Boldine affected both endoplasmic reticulum\u2010based IP\n                    <jats:sub>3<\/jats:sub>\n                    and ryanodine receptors (RyRs) and transmembrane calcium channels in NTR aortas, whereas in SHR aortas, its actions were linked to IP\n                    <jats:sub>3<\/jats:sub>\n                    and RyRs receptors. These findings indicate that boldine's vasorelaxant effects may contribute to vascular tone regulation through modulation of Ca\n                    <jats:sup>2<\/jats:sup>\n                    \u207a channels.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202501752","type":"journal-article","created":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T14:35:57Z","timestamp":1756132557000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Endothelium\u2010Independent Relaxation of Alkaloid Boldine in Isolated Aortas From Normotensive and Hypertensive Rats: Participation of Ca\n                    <sup>2+<\/sup>\n                    Channels"],"prefix":"10.1002","volume":"22","author":[{"given":"Martina Odebrecht","family":"Cavichiolo","sequence":"first","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences Universidade Do Vale Do Itaja\u00ed  Itaja\u00ed Santa Catarina Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita de C\u00e1ssia Vilhena","family":"da Silva","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences Universidade Do Vale Do Itaja\u00ed  Itaja\u00ed Santa Catarina Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valdir","family":"Cechinel Filho","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences Universidade Do Vale Do Itaja\u00ed  Itaja\u00ed Santa Catarina Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5251-5642","authenticated-orcid":false,"given":"Priscila","family":"de Souza","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Pharmaceutical Sciences Universidade Do Vale Do Itaja\u00ed  Itaja\u00ed Santa Catarina Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,25]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118829875.ch1"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2022.11.005"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehae466"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2018.07.008"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.36660\/abc.20201238"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcna.2017.04.007"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/10_2014_273"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/593672"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19071864"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1055\/a\u20102182\u20103665"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0000000000000185"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2012.11.010"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/4560607"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/FBP.0000000000000265"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.1267"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/9948707"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1998.0844"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2017.11.022"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12038\u2010011\u20109097\u2010y"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3109\/10641968009037159"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12646"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvs.2004.03.043"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2013.03.004"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161206778522056"},{"key":"e_1_2_10_26_1","volume-title":"Profiles of Drug Substances, Excipients and Related Methodology","author":"Al\u2010Majed A. 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