{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T10:33:06Z","timestamp":1791541986813,"version":"4.3.4"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:00:00Z","timestamp":1760918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:00:00Z","timestamp":1760918400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We aimed to integrate network pharmacology, metabonomics, and fecal transcriptomics to elucidate the mechanism of action of Diyu Shengbai Tablets (DST) in leukopenia treatment. Key bioactive components and core targets of DST in leukopenia were identified using network pharmacology. Spleen metabonomics, fecal transcriptomics, and MetOrigin analysis were employed to investigate potential mechanisms. Molecular docking, molecular dynamics simulations, and enzyme\u2010linked immunosorbent assay were performed to validate findings. Network pharmacology predicted Hypericin and Quercetin\u20103\u2010galactoside\u20107\u2010glucoside as major active constituents of DST. metabonomic analysis identified alterations in 133 differential metabolites in the spleen, with linoleic acid metabolism as the primary affected pathway. 16S rRNA sequencing indicated normalization of\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    abundance. Molecular docking and dynamics simulations showed low binding free energies between active compounds (e.g., Hypericin) and targets such as PIK3CA and SRC. Serum levels of Mouse interleukin\u20106 and Granulocyte colony\u2010stimulating factor were elevated following DST intervention. DST modulates spleen metabolic profiles in leukopenic mice, restores gut microbial composition, and exerts therapeutic effects by regulating linoleic acid metabolism and influencing the PI3K\u2010Akt signaling pathway. These findings provide a foundation for further exploration of multi\u2010target mechanisms in traditional Chinese medicine for leukopenia management.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202501772","type":"journal-article","created":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T16:29:46Z","timestamp":1760977786000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Unveiling the Mechanism Underlying Diyu Shengbai Tablet in Remediating Leukopenia Based on Network Pharmacology, Metabonomics, and Fecal Transcriptomics"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0932-7611","authenticated-orcid":false,"given":"Erbing","family":"Wang","sequence":"first","affiliation":[{"name":"College of Chemical Engineering and Technology Taiyuan University of Science and Technology  Taiyuan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Chemical Engineering and Technology Taiyuan University of Science and Technology  Taiyuan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingli","family":"Qu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Science Shanxi Medical University  Taiyuan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Chemical Engineering and Technology Taiyuan University of Science and Technology  Taiyuan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuying","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Chemical Engineering and Technology Taiyuan University of Science and Technology  Taiyuan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,20]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/08923973.2015.1128950"},{"key":"e_1_2_11_3_1","first-page":"60","article-title":"Analysis of Clinical Features of Leukopenia Patients in a Three Level General Hospital in Beijing","volume":"14","author":"Ye F.","year":"2017","journal-title":"China Medical Herald"},{"key":"e_1_2_11_4_1","first-page":"176","article-title":"Comparison of the Efficacy of Diyu Shengbai Tablets, Lixuesheng plus Shaganchun in the Treatment of Leukopenia","volume":"26","author":"Zhao X. J.","year":"2019","journal-title":"Capital Food & Medicine"},{"key":"e_1_2_11_5_1","first-page":"649","article-title":"Observation on Clinical Effect of Diyushengbai Tablets in the Prevention of Leukopenia in Patients with Non\u2010Small Cell Lung Cancer after Chemotherapy","volume":"25","author":"Ye Y. 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