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Extracts and fractions obtained after cultivation were analyzed by liquid chromatography coupled to high\u2010resolution mass spectrometry (LC\u2013HRMS) and compared using Global Natural Products Social molecular networking (GNPS), which revealed a family of seven putative polyunsaturated steroids. Isolation led to the characterization of two major steroids: the known Ergosta\u20103(4),5(6),7(8),14(15),22(23)\u2010pentaene (1a) and a previously unreported metabolite, Ergosta\u20105(6),7(8),14(15),22(23)\u2010tetraen\u20103\u2010ol (2a). Their structures were elucidated through comprehensive spectroscopic and spectrometric methods (e.g., UV\u2013Vis, NMR, and high\u2010resolution mass spectrometric [HRMS]), with the structure of steroid 2a being unequivocally confirmed by single\u2010crystal x\u2010ray diffraction (SCXRD). Notably, the biosynthesis of these steroids was significantly enhanced when the cultivation medium was supplemented with manganese chloride (MnCl\n                    <jats:sub>2<\/jats:sub>\n                    ). Furthermore, the steroids were tested for their in vitro antioxidant activity against the 2,2\u2010diphenyl\u20101\u2010picrylhydrazyl (DPPH) free radical using an adapted UHPLC\u2013DAD method, showing over 80% of the scavenging capacity of \u03b2\u2010carotene at the lowest tested concentrations.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202501816","type":"journal-article","created":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T20:06:41Z","timestamp":1763755601000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Manganese\u2010Induced Production of Antioxidant Polyene Steroids in the Extremophile Fungus\n                    <i>Talaromyces fuscoviridis<\/i>\n                    Isolated From Copper\u2010Mine Soil"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-1291-7913","authenticated-orcid":false,"given":"Mauricio Augusto Pinto Moreno da Silva","family":"Alves","sequence":"first","affiliation":[{"name":"Laboratory of Micromolecular Biochemistry of Microorganisms, Department of Chemistry Federal University of S\u00e3o Carlos  S\u00e3o Carlos S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5814-3624","authenticated-orcid":false,"given":"Alef dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Micromolecular Biochemistry of Microorganisms, Department of Chemistry Federal University of S\u00e3o Carlos  S\u00e3o Carlos S\u00e3o Paulo Brazil"},{"name":"Department of Chemistry Ume\u00e5 University  Ume\u00e5 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9175-3520","authenticated-orcid":false,"given":"Eduardo Jorge","family":"Pilau","sequence":"additional","affiliation":[{"name":"Laboratory of Biomolecules and Mass Spectrometry, Department of Chemistry State University of Maring\u00e1  Maring\u00e1 Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7841-8480","authenticated-orcid":false,"given":"Pedro Henrique de Oliveira","family":"Santiago","sequence":"additional","affiliation":[{"name":"Institute of Physics of S\u00e3o Carlos University of S\u00e3o Paulo  S\u00e3o Carlos S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0676-3098","authenticated-orcid":false,"given":"Javier Alcides","family":"Ellena","sequence":"additional","affiliation":[{"name":"Institute of Physics of S\u00e3o Carlos University of S\u00e3o Paulo  S\u00e3o Carlos S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilene Nunes","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Institute of Exact Sciences Federal University of the South and Southeast of Par\u00e1  Marab\u00e1 Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edson Rodrigues","family":"Filho","sequence":"additional","affiliation":[{"name":"Laboratory of Micromolecular Biochemistry of Microorganisms, Department of Chemistry Federal University of S\u00e3o Carlos  S\u00e3o Carlos S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.FUNK-0009-2016"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/21501203.2024.2354302"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/8860784"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.2003583"},{"key":"e_1_2_10_6_1","article-title":"Next\u2010Generation Sequencing Approach for Connecting Secondary Metabolites to Biosynthetic Gene Clusters in Fungi","volume":"5","author":"Cacho R. 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