{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T08:57:28Z","timestamp":1785488248604,"version":"3.56.0"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T00:00:00Z","timestamp":1769212800000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This study aimed to investigate\u00a0the therapeutic effects and molecular mechanisms of Modified Yiguanjian (MYGJ) in treating anti\u2010tuberculosis drug\u2010induced liver injury (ATB\u2010DILI). ATB\u2010DILI is a serious side effect of TB treatment, often leading to liver damage, drug resistance, or failure. The research\u00a0integrated\u00a0serum pharmacochemistry, network pharmacology, and metabolomics to uncover MYGJ's hepatoprotective mechanisms. Using ultra\u2010high\u2010performance liquid chromatography coupled with quadrupole\u2010electrostatic field orbitrap mass spectrometry, the study\u00a0identified\u00a011 prototype compounds of MYGJ in blood serum, including Apigenin and Lonicerin. The ATB\u2010DILI mouse model induced by isoniazid, rifampicin, and pyrazinamide\u00a0showed\u00a0MYGJ's effectiveness in reducing liver enzyme levels (alanine aminotransferase, aspartic transaminase, and alkaline phosphatase), improving histopathology, and lowering pro\u2010inflammatory cytokines (tumor necrosis factor\u2010\u03b1, interleukin [IL]\u20101\u03b2, and IL\u20106). Network pharmacology\u00a0identified\u00a0128 shared targets, including key molecules like TP53 and AKT1, and\u00a0suggested\u00a0tyrosine and purine metabolism as critical pathways. Metabolomics\u00a0identified\u00a095 altered metabolites, with MYGJ reversing 38 of them. Molecular docking\u00a0confirmed\u00a0strong binding between MYGJ compounds and targets like adenosine deaminase and myeloperoxidase. Overall, MYGJ\u00a0alleviated\u00a0ATB\u2010DILI through multi\u2010target, multi\u2010pathway actions, demonstrating its potential for liver protection in clinical settings.<\/jats:p>","DOI":"10.1002\/cbdv.202501824","type":"journal-article","created":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T11:19:47Z","timestamp":1769253587000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Elucidating the Mechanism of Modified Yiguanjian against Anti\u2010Tuberculosis Drug\u2010Induced Liver Injury via Integrated Pharmacology and Metabolomics"],"prefix":"10.1002","volume":"23","author":[{"given":"Yu","family":"Tu","sequence":"first","affiliation":[{"name":"College of Pharmacy Guangxi Medical University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingna","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Luocheng Mulam Autonomous County Traditional Chinese Medicine Hospital  Hechi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuting","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Basic Medicine Guangxi University of Traditional Chinese Medicine  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunxia","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Nanning Eighth People's Hospital  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Ou","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine HIV\/AIDS Clinical Treatment Center of Guangxi (Nanning) and The Fourth People's Hospital of Nanning  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangping","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Pharmacy Guangxi Medical University  Nanning 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