{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T08:57:27Z","timestamp":1785488247527,"version":"3.56.0"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T00:00:00Z","timestamp":1769385600000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Childhood obesity is a major preventable health issue. This study evaluated the anti\u2010obesity effects of Dahuang Huanglian Xiexin Decoction (DHXD) and explored its potential mechanisms using network pharmacology and molecular docking. Active compounds and targets of DHXD were identified from TCMSP and HERB databases, while obesity\u2010related targets were obtained from GeneCards, TTD, and DisGeNET. A protein\u2010protein interaction network helped identify 45 shared targets and 10 hub genes. Molecular docking confirmed strong binding between DHXD components and key targets. An obesity rat model was established using a high\u2010fat diet, followed by DHXD treatment. Parameters such as body weight, fat mass, and rectal temperature were monitored. Biochemical assays and histological staining assessed metabolic and tissue changes. Western blotting evaluated the expression of obesity\u2010related proteins. Results showed DHXD significantly reduced body weight and fat accumulation, improved blood glucose and lipid profiles, and promoted lipolysis and browning of white adipose tissue. Molecular analysis highlighted PPAR\u03b3, PTGS2, and STAT3 as central targets mediating DHXD's anti\u2010obesity effects. These findings support the potential of DHXD as an effective therapeutic agent against obesity and provide insights into its molecular mechanism of action.<\/jats:p>","DOI":"10.1002\/cbdv.202501831","type":"journal-article","created":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T09:06:11Z","timestamp":1769418371000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Dahuang Huanglian Xiexin Decoction Ameliorates Obesity by Targeting the PPAR\u03b3\/STAT3\/PTGS2 Axis: Integrative Network Pharmacology and Experimental Validation"],"prefix":"10.1002","volume":"23","author":[{"given":"Huan","family":"Gu","sequence":"first","affiliation":[{"name":"Department of Integrative Cardiology China\u2010Japan Friendship Hospital Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lichang","family":"Yang","sequence":"additional","affiliation":[{"name":"Graduate School Nanjing University of Chinese Medicine Nanjing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Beijing Fangshan District Liangxiang Hospital  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Beijing Fangshan District Liangxiang Hospital  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5678-5919","authenticated-orcid":false,"given":"Ji","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Guang'anmen Hospital Chinese Academy of Traditional Chinese Medicine  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,26]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.120.316101"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.23373"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/obr.12649"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2020.08.016"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.01285"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2019.10.013"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.1085092"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.2147\/dmso.S517874"},{"issue":"6","key":"e_1_2_11_10_1","first-page":"336","article-title":"The Ancient Doctor's View on Treatment of Fat","volume":"23","author":"Gong H. Z. H.","year":"2004","journal-title":"Beijing Journal of TCM"},{"key":"e_1_2_11_11_1","volume-title":"Effect of Dahuang Huanglian Xiexin Decotion on Insulin Resistance and GLUT4 Protein Expression of Skeletal Muscle in Mice of Type 2 Diabetes","author":"Mi S. Q. J.","year":"2017"},{"key":"e_1_2_11_12_1","volume-title":"Study on the Effect of Jiangzhuo Recipe for High Density Lipoprotein and Uric Acid in Patients With Dyslipidemia With Hyperlipidemia","author":"Jinsong Liu C. L.","year":"2017"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0254\u20106272(16)30042\u20105"},{"issue":"9","key":"e_1_2_11_14_1","first-page":"1441","article-title":"The Mechanism of Dahuang Huanglian Xiexin Decoction in Treating Sepsis\u2010induced Myocardial Dysfunction Based on Network Pharmacology","volume":"19","author":"Qiyu Liu Q. L.","year":"2021","journal-title":"Chinese Journal of Integrative Medicine on Cardio\u2010Cerebrovascular Disease"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.112032"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prostaglandins.2024.106874"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.21037\/apm-19-271"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.956313"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26146678"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2017.12.001"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2021.11.004"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/9780677"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compbiomed.2022.105389"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/1758\u20102946\u20106\u201013"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1063"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1049"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/database\/baq020"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab953"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz1021"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw937"},{"key":"e_1_2_11_31_1","doi-asserted-by":"crossref","unstructured":"L.Dai G.Zhang andX.Wan.Network Pharmacology and Molecular Docking Analysis Reveal Insights into the Molecular Mechanism of Shengma\u2010Gegen Decoction on Monkeypox. (2022).","DOI":"10.3390\/pathogens11111342"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201036687\u2010z"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.631982"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21334"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1159\/000308466"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/ecam\/nep124"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2012.11.008"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00955"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio.12.81"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2017.06.123"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12944\u2010022\u201001662\u20106"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2022.175040"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3361"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2020.105291"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2020.08.016"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.69054"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867325666180514112124"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.47746"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202501831","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.202501831","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202501831","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T07:59:05Z","timestamp":1785484745000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202501831"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":48,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1002\/cbdv.202501831"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202501831","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-05-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01831"}}