{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T09:58:58Z","timestamp":1790848738348,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T00:00:00Z","timestamp":1758067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T00:00:00Z","timestamp":1758067200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A new triterpenoid saponin designated as Sigmastane, 5(6), 9(11), 22(23), E, triene\u20103\u2010O\u2010\u03b2\u2010glycoside, along with a mixture of known compounds, was isolated from the aerial part of the methanol extract of\n                    <jats:italic>Tridax procumbens<\/jats:italic>\n                    L. The structure of the compound was elucidated through one and two\u2010dimensional nuclear magnetic resonance and high\u2010resolution electrospray ionization mass spectrometry. The in vitro cytotoxic potential of the isolated compound was evaluated against two human cancer cell lines, MCF\u20107 and MDA\u2010MB\u2010249, using doxorubicin as a standard. The saponin displayed strong cytotoxic effects across both tested cell lines (MCF\u20107 and MDA\u2010MB\u2010249 cell lines, yielding half\u2010maximal inhibitory concentration (IC\n                    <jats:sub>50<\/jats:sub>\n                    ) values of 6.55 and 3.96\u00a0\u00b5g\/mL, respectively). Additionally, the antioxidant activity of the compound was evaluated using the DPPH assay, resulting in an IC\n                    <jats:sub>50<\/jats:sub>\n                    value of 1.46\u00a0\u00b5g\/mL. In\u2010vitro antibacterial activity showed significant minimum inhibitory concentration (MIC) at 62.5 and 250\u00a0\u00b5g\/mL against\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    and\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    , respectively. The compound showed activity against\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    (vaccine strain, H37RV strain) with MIC = 50 \u00b5g\/mL. The molecular docking study for\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    and breast cancer cell lines targeted proteins of\n                    <jats:bold>1a<\/jats:bold>\n                    exhibited a binding energy range of \u221210.1 to \u22127.4\u00a0kcal\/mol. These isolated saponins may serve as promising drug candidates for future research and the development in the pharmaceutical industry.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202501842","type":"journal-article","created":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T20:18:36Z","timestamp":1758140316000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A New Saponin From\n                    <i>Tridax procumbens<\/i>\n                    L.: In Vitro and In Silico Studies Reveal Broad Spectrum of Bioactivities"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8255-1628","authenticated-orcid":false,"given":"Varsharani V.","family":"Ingole","sequence":"first","affiliation":[{"name":"Department of Chemistry STES's Sinhgad College of Science Savitribai Phule Pune University  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sushma R.","family":"Katade","sequence":"additional","affiliation":[{"name":"Department of Chemistry PES's Modern College of Arts, Science and Commerce Savitribai Phule Pune University  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pravin C.","family":"Mhaske","sequence":"additional","affiliation":[{"name":"Post\u2010Graduate Department of Chemistry S. P. Mandali's, Sir Parshurambhau College, Savitribai Phule Pune University  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siraj B.","family":"Shaikh","sequence":"additional","affiliation":[{"name":"Department of Chemistry MCE's Abeda Inamdar College of Arts, Science and Commerce Savitribai Phule Pune University  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cis.2020.102145"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcis.2018.10.024"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(02)00393-X"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2010.06.004"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/109662004322984734"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN2002725"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules21101404"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2006.10.008"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2004.05.016"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11101-005-3852-3"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2011.01.015"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.3c10439"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.2307\/2805391"},{"key":"e_1_2_9_15_1","article-title":"Influence of Tridax procumbens on Dead Space Wound Healing","author":"Udupa S. 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