{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T01:47:05Z","timestamp":1789523225533,"version":"build-2803163510"},"reference-count":64,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T00:00:00Z","timestamp":1764720000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T00:00:00Z","timestamp":1764720000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study investigates the phytochemical composition and pharmacological potential of\n                    <jats:italic>Verbascum thapsus<\/jats:italic>\n                    L. leaf extracts. Cold maceration with solvents of increasing polarity (hexane, ethyl acetate, ethanol, and methanol) yielded extracts rich in phenolics, flavonoids, and tannins, as confirmed by phytochemical screening and Fourier\u2010transform infrared (FTIR) detection of aldehydes, ketones, and aliphatic and aromatic groups. LC\u2013MS analysis further identified key bioactive constituents, including daidzein, ellagic acid, sinapinic acid, and scutellarin. Among the tested extracts, polar solvents exhibited the strongest antioxidant activity. The methanol extract showed notable activity in the DPPH assay (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a0275.16\u00a0\u00b1\u00a014.68\u00a0\u00b5g\/mL) and ferric\u2010reducing antioxidant power (FRAP) assay (302.82\u00a0\u00b1\u00a026.39\u00a0\u00b5M), while the ethanol extract also demonstrated strong activity in both the DPPH assay (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a0303.31\u00a0\u00b1\u00a02.64\u00a0\u00b5g\/mL) and FRAP assay (334.75\u00a0\u00b1\u00a013.12\u00a0\u00b5M). In antibacterial assessments, the ethanolic extract was most effective, exhibiting significant potency against\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    with an inhibition zone of 16.8\u00a0mm and a minimum inhibitory concentration (MIC) of 31.5\u00a0\u00b5g\/mL. Taken together, these findings demonstrate that the remarkable bioactivities of\n                    <jats:italic>V. thapsus<\/jats:italic>\n                    are directly linked to its rich profile of antioxidant and antimicrobial phytochemicals, strongly supporting its potential as a valuable source of natural therapeutic agents.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202501856","type":"journal-article","created":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T20:18:51Z","timestamp":1764793131000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Metabolic Profiling, Antioxidant, and Antibacterial Potential of Himalayan\n                    <i>Verbascum thapsus<\/i>\n                    L."],"prefix":"10.1002","volume":"23","author":[{"given":"Aarif","family":"Nazir","sequence":"first","affiliation":[{"name":"Department of Zoology, Advanced Research Lab University of Kashmir  India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ishteyaq Majeed","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Zoology, Advanced Research Lab University of Kashmir  India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fayaz","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Zoology, Advanced Research Lab University of Kashmir  India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaista","family":"Masarat","sequence":"additional","affiliation":[{"name":"Hydrobiology Lab S.P College Srinagar  Srinagar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashaq Sultan","family":"Dar","sequence":"additional","affiliation":[{"name":"Department of Zoology, Advanced Research Lab University of Kashmir  India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5981-7524","authenticated-orcid":false,"given":"Ibraq","family":"Khurshid","sequence":"additional","affiliation":[{"name":"Department of Zoology Central University of Kashmir  Ganderbal 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