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Essential oils derived from\n                    <jats:italic>Eugenia<\/jats:italic>\n                    species are known for their medicinal properties and have been studied for their antimicrobial and bioactive potential. This study aimed to evaluate, through in vitro tests, the antibacterial, antibiofilm, and antibiotic\u2010modulating effects of\n                    <jats:italic>Eugenia brejoensis<\/jats:italic>\n                    essential oil (EOEb) against multidrug\u2010resistant (MDR) clinical isolates of\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    and\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    from COVID\u201019 patients. In addition, we sought to analyze its toxicity and survival rates through in vivo tests in an invertebrate model using\n                    <jats:italic>Tenebrio molitor<\/jats:italic>\n                    . The EOEb was extracted via hydrodistillation and analyzed by gas chromatography\u2013mass spectrometry (GC\u2013MS) and flame ionization detector (FID). Clinical isolates of\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    and\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    were identified using matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry (MALDI\u2013TOF MS), and their resistance profiles were determined using the Vitek 2 system. The in vitro tests were conducted using the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using microdilution and plating methods. Biofilm formation and inhibition assays were performed using crystal violet staining. Synergistic effects of EOEb with ciprofloxacin and gentamicin were evaluated using the checkerboard assay. The antibacterial effect of EOEb was tested in vivo using the\n                    <jats:italic>T. molitor<\/jats:italic>\n                    (mealworm) larvae model to assess toxicity and survival rates. The major constituents of EOEb were rosifoliol (16.47%), guaiol (12.69%), and (\n                    <jats:italic>E<\/jats:italic>\n                    )\u2010caryophyllene (11.97%). All bacteria exhibited an MDR profile. EOEb showed significant antibacterial activity against MDR strains, with MIC and MBC values ranging from 0.512 to 4.096\u00a0mg\/mL. It also effectively inhibited biofilm formation at concentrations between 0.512 and 4.096\u00a0mg\/mL. EOEb exhibited synergistic effects with ciprofloxacin against\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    and with gentamicin against\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    , as indicated by fractional inhibitory concentration (FIC) indices close to 0.5. The EOEb demonstrated low toxicity in the\n                    <jats:italic>T. molitor<\/jats:italic>\n                    model, with a survival rate of approximately 70%. The EOEb exhibits notable antimicrobial and biofilm\u2010inhibiting properties against MDR pathogens. Its low toxicity and synergistic effects with conventional antibiotics suggest its potential as a therapeutic alternative for combating antibiotic\u2010resistant infections.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202501906","type":"journal-article","created":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T21:45:46Z","timestamp":1762379146000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Potential of\n                    <i>Eugenia brejoensis<\/i>\n                    (Mazine) Essential Oil in Combating Multidrug\u2010Resistant (MDR)\n                    <i>Acinetobacter baumannii<\/i>\n                    and\n                    <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1002","volume":"23","author":[{"given":"Jonathan Mand\u00fa","family":"de Ara\u00fajo","sequence":"first","affiliation":[{"name":"Federal University of Pernambuco  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda Vieira","family":"de Barros","sequence":"additional","affiliation":[{"name":"Federal University of Pernambuco  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1bio Henrique Galdino Dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Federal University of Pernambuco  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weslley Felix","family":"De Oliveira","sequence":"additional","affiliation":[{"name":"Federal University of Pernambuco  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela Maria","family":"do Amaral Ferraz Navarro","sequence":"additional","affiliation":[{"name":"Federal University of Pernambuco  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rcia Vanusa","family":"da Silva","sequence":"additional","affiliation":[{"name":"Federal University of Pernambuco  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno Oliveira","family":"de Veras","sequence":"additional","affiliation":[{"name":"Federal University of Pernambuco  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John Eversong Lucena","family":"de Vasconcelos","sequence":"additional","affiliation":[{"name":"CECAPE College  Juazeiro do Norte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6634-4207","authenticated-orcid":false,"given":"Henrique Douglas Melo","family":"Coutinho","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry Regional University of Cariri \u2013 URCA  Crato Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Bet\u00e2nia","family":"de Melo Oliveira","sequence":"additional","affiliation":[{"name":"Federal University of Pernambuco  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Tereza","family":"dos Santos Correia","sequence":"additional","affiliation":[{"name":"Federal University of Pernambuco  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/0972060x.2014.1000390"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00424"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.999131"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/jfb13030149"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2024.107078"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666\u20105247(22)00355\u2010X"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2023.12.009"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2024.106183"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.26633\/RPSP.2019.65"},{"key":"e_1_2_10_11_1","volume-title":"WHO Bacterial Priority Pathogens List, 2024: Bacterial Pathogens of Public Health Importance to Guide Research, Development and Strategies to Prevent and Control Antimicrobial Resistance","author":"WHO","year":"2024"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/10412905.2020.1746698"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26030628"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2021.153626"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2020.1846494"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12071191"},{"key":"e_1_2_10_17_1","doi-asserted-by":"crossref","unstructured":"H. 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