{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:36:00Z","timestamp":1787319360768,"version":"build-2736575974"},"reference-count":61,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T00:00:00Z","timestamp":1763164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T00:00:00Z","timestamp":1763164800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study evaluated the extraction efficiency, phenolic composition, and bioactivity of\n                    <jats:italic>Cachrys cristata<\/jats:italic>\n                    using different methods. Ultrasound\u2010assisted extraction (US) yielded higher extract amounts and phenolic contents, including flavonoids and flavonols, compared to Soxhlet extraction (S). While US 70% ethanol gave the highest extract yield, the US methanol (MeOH) extract contained the highest total phenolic content (105.47\u00a0mg gallic acid equivalents\/g dry extract) and was selected for LC\u2013MS profiling. Seventeen compounds were identified, with gentisic acid (39.03\/50.73\u00a0mg\/L), ferulic acid (27.00\/25.68\u00a0mg\/L), and rutin (13.28\/11.91\u00a0mg\/L) as dominant in Soxhlet and US extracts, respectively. The US MeOH extract showed strong antioxidant activity in DPPH and ABTS assays and eightfold higher anti\u2010tyrosinase activity than kojic acid, while no acetylcholinesterase inhibition was observed. The hexane extract exhibited potent antibacterial effects against\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    ,\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    , and\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    , inhibited biofilm formation and pyocyanin in\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    , and had limited antifungal activity. It also strongly inhibited bovine serum albumin denaturation (88.88% vs. 95.60% for diclofenac). For antidiabetic potential, the US methylene chloride extract inhibited \u03b1\u2010amylase better than acarbose (IC\n                    <jats:sub>50<\/jats:sub>\n                    0.267 vs. 0.832\u00a0mg\/mL) and moderately reduced \u03b1\u2010glucosidase activity. These results provide novel insights into the phenolic profile and multifunctional bioactivity of\n                    <jats:italic>C. cristata<\/jats:italic>\n                    extracts.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202501910","type":"journal-article","created":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T17:20:20Z","timestamp":1763227220000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparative LC\u2013MS Analysis and In Vitro Evaluation of Antioxidant, Antimicrobial, and Enzyme Inhibitory Properties of Ultrasound\u2010Assisted and Soxhlet Extracts of\n                    <i>Cachrys cristata<\/i>\n                    DC."],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6116-5384","authenticated-orcid":false,"given":"Ksenija S.","family":"Mileski","sequence":"first","affiliation":[{"name":"Department of Morphology and Systematics of Plants Faculty of Biology, Institute of Botany and Botanical Garden \u201cJevremovac\u201d, University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana D.","family":"\u0106iri\u0107","sequence":"additional","affiliation":[{"name":"Department of Plant Physiology Institute for Biological Research \u201cSinis\u030ca Stankovic\u0301\u201d, University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lazar D.","family":"\u017darkovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Morphology and Systematics of Plants Faculty of Biology, Institute of Botany and Botanical Garden \u201cJevremovac\u201d, University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uro\u0161 M.","family":"Ga\u0161i\u0107","sequence":"additional","affiliation":[{"name":"Department of Plant Physiology Institute for Biological Research \u201cSinis\u030ca Stankovic\u0301\u201d, University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petar D.","family":"Marin","sequence":"additional","affiliation":[{"name":"Department of Morphology and Systematics of Plants Faculty of Biology, Institute of Botany and Botanical Garden \u201cJevremovac\u201d, University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana M.","family":"D\u017eami\u0107","sequence":"additional","affiliation":[{"name":"Department of Morphology and Systematics of Plants Faculty of Biology, Institute of Botany and Botanical Garden \u201cJevremovac\u201d, University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,15]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"346","volume-title":"Flora of the Republic of Serbia","author":"Nikoli\u0107 V.","year":"1973"},{"key":"e_1_2_9_3_1","first-page":"343","volume-title":"Flora Europaea","author":"Tutin T. 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