{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T19:19:01Z","timestamp":1790018341557,"version":"4.0.1"},"reference-count":64,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T00:00:00Z","timestamp":1762473600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T00:00:00Z","timestamp":1762473600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    An ever\u2010increasing number of ailments are being treated using\n                    <jats:italic>Hypericum<\/jats:italic>\n                    L. species, which are gaining both popular and scientific relevance. This study set out to thoroughly examine the chemical and biological aspects of ethanol extracts from the roots and aerial parts of the\n                    <jats:italic>Hypericum empetrifolium<\/jats:italic>\n                    subsp.\n                    <jats:italic>empetrifolium<\/jats:italic>\n                    Willd.,\n                    <jats:italic>Hypericum triquetrifolium<\/jats:italic>\n                    Turra, and\n                    <jats:italic>Hypericum pruinatum<\/jats:italic>\n                    Boiss. Et. Honey species. Thus, it is aimed to shed light on the uses of\n                    <jats:italic>Hypericum<\/jats:italic>\n                    species among the public by revealing both their chemical and biological profiles. Species\u2010specific phenolic content of the samples was determined by LC\u2013MS\/MS. In addition, antioxidant, anticholinesterase, urease, tyrosinase, elastase, collagenase, and angiotensin I\u2010converting enzyme (ACE) inhibition activities of the extracts and some major components (chlorogenic acid, hesperidin, hyperoside, quercitrin, quercetin, pseudohypericin, hypericin) specific to\n                    <jats:italic>Hypericum<\/jats:italic>\n                    species were determined. LC\u2013MS\/MS results showed that some samples were rich in hyperoside (49\u00a0365.6\u00a0\u00b5g analyte\/g extract), hesperidin (10\u00a0077.8), chlorogenic acid (15\u00a0027.8), luteolin\u20107\u2010glycoside (1629.6), quercitrin (6979.7), pseudohypericin (998.8), and hypericin (386.5). The samples were found to have a high potential in CUPRAC, DPPH, and ABTS antioxidant methods. High enzyme activity was seen in particular for acetylcholinesterase (AChE) (inhibition%: 54.00\u00a0\u00b1\u00a00.97)\n                    <jats:italic>H. pruinatum<\/jats:italic>\n                    root ethanol extract, for butyrylcholinesterase (BChE) (inhibition%: 73.33\u00a0\u00b1\u00a01.49) of\n                    <jats:italic>H. triquetrifolium<\/jats:italic>\n                    aerial part ethanol extract, for urease (inhibition%: 49.20\u00a0\u00b1\u00a01.27) of\n                    <jats:italic>H. empetrifolium<\/jats:italic>\n                    subsp\n                    <jats:italic>. empetrifolium<\/jats:italic>\n                    aerial part ethanol extract, for tyrosinase (inhibition%: 55.40\u00a0\u00b1\u00a00.52) of\n                    <jats:italic>H. pruinatum<\/jats:italic>\n                    root ethanol extract, and elastase (inhibition%: 36.84\u00a0\u00b1\u00a00.45) of\n                    <jats:italic>H. pruinatum<\/jats:italic>\n                    aerial part ethanol extract. The enzyme inhibitory potentials of hesperidin (urease inhibition%: 54.32\u00a0\u00b1\u00a01.35, tyrosinase: 73.87\u00a0\u00b1\u00a02.06, elastase: 82.23\u00a0\u00b1\u00a01.41, collagenase: 67.37\u00a0\u00b1\u00a00.73, and ACE: 80.74\u00a0\u00b1\u00a00.85) and quercetin (AChE inhibition%: 57.82\u00a0\u00b1\u00a01.21, BChE: 92.82\u00a0\u00b1\u00a01.84, urease: 73.17\u00a0\u00b1\u00a01.79, tyrosinase: 65.25\u00a0\u00b1\u00a01.73, elastase: 98.04\u00a0\u00b1\u00a02.28, and ACE: 86.74\u00a0\u00b1\u00a00.85), principal constituents of\n                    <jats:italic>Hypericum<\/jats:italic>\n                    species, were shown to be significantly high on the basis of both in vitro and in silico analyses. The in vitro and in silico test findings of\n                    <jats:italic>Hypericum<\/jats:italic>\n                    species and their principal components indicate that they are promising sources of natural agents, suggesting their potential application in the food and pharmaceutical industries.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202501956","type":"journal-article","created":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T20:35:24Z","timestamp":1762547724000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In Vitro and In Silico Studies on Unique Key Components of\n                    <i>Hypericum<\/i>\n                    Species, Focusing on Their Role in Inhibiting Enzymatic Pathways"],"prefix":"10.1002","volume":"23","author":[{"given":"Serkan","family":"Yigitkan","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy Dicle University  Diyarbakir 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