{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T16:00:41Z","timestamp":1785081641297,"version":"3.55.0"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:00Z","timestamp":1778544000000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Orientin is a water\u2010soluble flavonoid C\u2010glycoside present in various medicinal plants and known for its diverse pharmacological activities. The present study aimed to evaluate the acute antinociceptive effects of orientin and to investigate the involvement of adrenergic, opioidergic, cannabinoid systems, and ATP\u2010sensitive potassium channels in these effects. Orientin (50, 100, and 200\u00a0mg\/kg, i.p.) was administered to mice. Central antinociceptive activity was assessed using the hot\u2010plate and tail\u2010immersion tests, while peripheral activity was evaluated using the acetic acid\u2010induced writhing test. To explore the underlying mechanisms, mice were pretreated with yohimbine (\u03b12\u2010adrenoceptor antagonist), naloxone (\u03bc\u2010opioid receptor antagonist), AM251 (CB1 receptor antagonist), AM630 (CB2 receptor antagonist), or glibenclamide (KATP channel blocker) prior to orientin administration. Orientin significantly reduced nociceptive responses in all experimental models at the tested doses. All antagonists except glibenclamide significantly reversed the spinal, supraspinal, and peripheral antinociceptive effects of orientin to varying degrees. These findings suggest that \u03b12\u2010adrenoceptors, \u03bc\u2010opioid receptors, and cannabinoid CB1 and CB2 receptors contribute to the analgesic effect of orientin, whereas KATP channels do not appear to be directly involved.<\/jats:p>","DOI":"10.1002\/cbdv.202501995","type":"journal-article","created":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T06:46:07Z","timestamp":1778654767000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Vivo Evaluation of the Analgesic Effects of Orientin With Possible Underlying Mechanisms"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2360-511X","authenticated-orcid":false,"given":"Hazal","family":"Eken","sequence":"first","affiliation":[{"name":"Department of Pharmacology Faculty of Pharmacy Afyonkarahisar Health Sciences University  Afyonkarahisar Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8041-6844","authenticated-orcid":false,"given":"Rana","family":"Arslan","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Faculty of Pharmacy Anadolu University  Eskisehir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9146-3841","authenticated-orcid":false,"given":"Feyza Alyu","family":"Alt\u0131nok","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Faculty of Pharmacy Anadolu University  Eskisehir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2597-6184","authenticated-orcid":false,"given":"Nurcan","family":"Bektas","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Faculty of Pharmacy Anadolu University  Eskisehir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000001939"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.15386\/cjmed\u2010413"},{"key":"e_1_2_8_4_1","first-page":"1","article-title":"Effects of Passiflora cincinnata in Murine Models of Nociception","volume":"79","author":"do Nascimento Sales E. 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