{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T15:31:04Z","timestamp":1787585464790,"version":"build-2736575974"},"reference-count":87,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T00:00:00Z","timestamp":1760313600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T00:00:00Z","timestamp":1760313600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The emergence of antimicrobial\u2010resistant bacteria has significantly compromised global public health. The World Health Organization has identified priority pathogens for research and new therapeutic strategies, highlighting carbapenem\u2010resistant\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    as a critical priority.\n                    <jats:italic>Ambrosia hispida<\/jats:italic>\n                    essential oil (EO) from the Yucatan Peninsula was investigated for its anti\u2010\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    properties. GC\u2013MS analysis identified 27 metabolites, predominantly sesquiterpenes and oxygenated sesquiterpenes, with spathulenol, caryophyllene oxide, and \u03b1\u2010pinene as major compounds. This EO exhibited antibacterial activity against\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    strains, strong inhibition of biofilm formation, and anti\u2010quorum\u2010sensing activity. Moreover, the EO also decreased bacterial survival in response to H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    , indicating anti\u2010catalase activity. Scanning electron microscopy (SEM) analysis confirmed the EO's ability to inhibit biofilm formation and cause morphological alterations in bacterial cells. Molecular docking studies revealed that kaurene, a diterpene in the EO, had the strongest interaction with bacterial targets involved in biofilm formation. The study highlights the potential of\n                    <jats:italic>A. hispida<\/jats:italic>\n                    EO as a promising candidate for developing agents to combat carbapenem\u2010resistant\n                    <jats:italic>A<\/jats:italic>\n                    <jats:italic>. baumannii<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202502050","type":"journal-article","created":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T05:02:34Z","timestamp":1760418154000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Mechanistic Insights Into the Effect of the Essential Oil of\n                    <i>Ambrosia hispida<\/i>\n                    Pursh. Against Multidrug\u2010Resistant Clinical Isolates of\n                    <i>Acinetobacter baumannii<\/i>"],"prefix":"10.1002","volume":"22","author":[{"given":"Haziel Eleazar","family":"Dzib\u2010Baak","sequence":"first","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica Yucat\u00e1n Instituto Mexicano del Seguro Social  M\u00e9rida M\u00e9xico"},{"name":"Unidad de Recursos Naturales, Centro de Investigaci\u00f3n Cient\u00edfica De Yucat\u00e1n  M\u00e9rida M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3961-6449","authenticated-orcid":false,"given":"Gloria Mar\u00eda","family":"Molina\u2010Salinas","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica Yucat\u00e1n Instituto Mexicano del Seguro Social  M\u00e9rida M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s Humberto","family":"Uc\u2010Cach\u00f3n","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica Yucat\u00e1n Instituto Mexicano del Seguro Social  M\u00e9rida M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio Nemorio","family":"Hidalgo\u2010Figueroa","sequence":"additional","affiliation":[{"name":"SECIHTI\u2010Divisi\u00f3n de Biolog\u00eda Molecular Instituto Potosino de Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica A.C.  San Luis Potos\u00ed M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1ngel","family":"Dzul\u2010Beh","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica Yucat\u00e1n Instituto Mexicano del Seguro Social  M\u00e9rida M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Javier","family":"Quintal\u2010Novelo","sequence":"additional","affiliation":[{"name":"Unidad M\u00e9dica de Alta Especialidad, Centro M\u00e9dico Ignacio Garc\u00eda T\u00e9llez Instituto Mexicano del Seguro Social  M\u00e9rida M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas Alfredo","family":"Araujo\u2010Le\u00f3n","sequence":"additional","affiliation":[{"name":"Unidad de Biolog\u00eda Integrativa Centro de Investigaci\u00f3n Cient\u00edfica de Yucat\u00e1n  M\u00e9rida M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5161-7823","authenticated-orcid":false,"given":"Luz Mar\u00eda","family":"Calvo\u2010Irabien","sequence":"additional","affiliation":[{"name":"Unidad de Recursos Naturales, Centro de Investigaci\u00f3n Cient\u00edfica De Yucat\u00e1n  M\u00e9rida M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,13]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"3251","article-title":"Enfermedades infecciosas. Concepto, clasificaci\u00f3n, aspectos generales y espec\u00edficos de las infecciones","volume":"10","author":"Palomo J.","year":"2010","journal-title":"Medicine"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469\u20100691.2011.03570.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S234610"},{"key":"e_1_2_9_5_1","unstructured":"World Health Organization WHO Bacterial Priority Pathogens List: Bacterial Pathogens of Public Health Importance to Guide Research Development and Strategies to Prevent and Control Antimicrobial Resistance 1st ed. (World Health Organization 2024)."},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10030373"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9040205"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpt.13787"},{"key":"e_1_2_9_9_1","first-page":"56","article-title":"Antimicrobial Importance of Medicinal Plants in Nigeria","volume":"32","author":"Ugboko H. U.","year":"2020","journal-title":"Scientific World Journal"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061594"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.2174\/2212796817666221208115047"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.114369"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0277\u20109536(98)00181\u20106"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20108741(01)00355\u20105"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26154701"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2020.110305"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874285801408010006"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2019.104433"},{"key":"e_1_2_9_19_1","unstructured":"J. A.Arellano Rodr\u00edguez J. S.Flores Guido J.Tun\u2010Garrido andM. C.Bojorquez Nomenclatura y forma de vida uso manejo y distribuci\u00f3n de las especies vegetales de la pen\u00ednsula de Yucat\u00e1n(Universidad Aut\u00f3noma de Yucat\u00e1n 2003)."},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20108741(02)00002\u20108"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/10412905.2005.9698917"},{"key":"e_1_2_9_22_1","first-page":"1153","article-title":"In Silico Polypharmacology of Natural Products","volume":"19","author":"Fang J.","year":"2018","journal-title":"Briefings in Bioinformatics"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751\u20107915.13945"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13099\u2010021\u201000443\u20103"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.201300389"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ffj.1875"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjp.2019.06.001"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2015.12.008"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11696\u2010021\u201001865\u20108"},{"key":"e_1_2_9_30_1","doi-asserted-by":"crossref","DOI":"10.1177\/1934578X1000500906","article-title":"Antimicrobial Activities of Single Aroma Compounds","volume":"5","author":"Schmidt E.","year":"2010","journal-title":"Natural Products Communications"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules200711808"},{"key":"e_1_2_9_32_1","first-page":"11","article-title":"Potential Antibacterial Action of \u03b1\u2010Pinene","volume":"12","author":"Borges M. F. D. A.","year":"2022","journal-title":"Medical Sciences Forum"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.989667"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1080\/0972060X.2023.2179424"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules17066305"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2013.09.001"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/np100472d"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.124843"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph16010055"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2015.1004673"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2021.1960521"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2015.1110705"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics10121474"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms7090345"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2012.01.045"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph6121451"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/scipharm91030033"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27051484"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.27369"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2017.10.021"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201079128\u2010x"},{"key":"e_1_2_9_52_1","first-page":"101","volume-title":"Quorum Sensing vs Quorum Quenching: A Battle With no End in Sight","author":"Bhargava N.","year":"2014"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20108741(03)00144\u20102"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics13030327"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11010108"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2023.106473"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41522\u2010023\u201000465\u20106"},{"key":"e_1_2_9_58_1","first-page":"3567","article-title":"Acinetobacter baumannii Biofilm: Intervening Factors, Persistence, Drug Resistance, and Strategies of Treatment","volume":"9","author":"Elkheloui R.","year":"2020","journal-title":"Mediterranean Journal of Infection, Microbes and Antimicrobials"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00096\u201009"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781003097358-12"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pch149"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2015.04.116"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.14421\/biomedich.2023.121.151-157"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29194631"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1590\/1414\u2010431X20121423"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786410802267734"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201072740\u20101"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096\u2010020\u201003882\u2010z"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crmicr.2022.100131"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26541\u20100"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11070870"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2022.10.095"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000058"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929\u2010020\u20100617\u20107"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1549"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-177-2_19"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.2174\/157340911795677602"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsos.201652"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010025\u201088191\u20101"},{"key":"e_1_2_9_80_1","first-page":"456","volume-title":"Identification of Essential Oil Components by Gas Chromatography\/Mass Spectrometry","author":"Adams R. P.","year":"2007"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.3653552"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906\u2010020\u20102837\u20105"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9110730"},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e31420"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.17113\/ftb.57.02.19.5823"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms252413241"},{"key":"e_1_2_9_87_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jcim.1c00203"},{"key":"e_1_2_9_88_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21334"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202502050","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.202502050","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202502050","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T11:50:11Z","timestamp":1773489011000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202502050"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,13]]},"references-count":87,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/cbdv.202502050"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202502050","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,13]]},"assertion":[{"value":"2025-06-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02050"}}