{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T07:02:27Z","timestamp":1775890947858,"version":"3.50.1"},"reference-count":64,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The current study is aimed to reveal the phytoprofile of\n                    <jats:italic>Selaginella inaequalifolia<\/jats:italic>\n                    (Hook. &amp; Grev.) Spring using GC\u2013MS and predict the drug properties, toxicity, biological properties of\n                    <jats:italic>S. inaequalifolia<\/jats:italic>\n                    ethanolic extracts (\n                    <jats:italic>Si<\/jats:italic>\n                    EE) using in silico methods and in vitro toxicity assays, namely, MTT and BSLB assay. A total of 27 compounds are identified from\n                    <jats:italic>Si<\/jats:italic>\n                    EE with varied physicochemical properties. Various biological properties of the identified compound are predicted based on online PASS prediction. The ADME and toxicity profile analysis identified pentadecanoic acid, 13\u2010methyl\u2010, methyl ester as a strong CDK2 and mTOR inhibitor, suggesting anticancer potential. A dose\u2010dependent toxicity and cytotoxicity are observed. The brine shrimp lethality assay (LC\n                    <jats:sub>50<\/jats:sub>\n                    : 274.26\u00a0mg\/mL) indicated low cytotoxicity, while MCF\u20107 breast cancer cell line studies (IC\n                    <jats:sub>50<\/jats:sub>\n                    : 42.49\u00a0\u00b5g\/mL) showed promising anticancer activity. These findings support\n                    <jats:italic>S. inaequalifolia<\/jats:italic>\n                    as a potential source of therapeutic agents, warranting further molecular and clinical investigations.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202502119","type":"journal-article","created":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T12:38:38Z","timestamp":1764419918000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chemical Profiling, In Silico and In Vitro Studies to Identify Potential CDK2 and mTOR Inhibitor From\n                    <i>Selaginella inaequalifolia<\/i>\n                    (Hook. &amp; Grev.) Spring Ethanolic Extracts"],"prefix":"10.1002","volume":"23","author":[{"given":"Johnson Marimuthu Alias","family":"Antonysamy","sequence":"first","affiliation":[{"name":"Centre for Plant Biotechnology, Department of Botany St. Xavier's College (Autonomous)  Palayamkottai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sivaraman","family":"Arumugam","sequence":"additional","affiliation":[{"name":"Centre for Plant Biotechnology, Department of Botany St. Xavier's College (Autonomous)  Palayamkottai India"},{"name":"Maria Research Foundation Alagar Nagar  Tirunelveli India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin Charles Antony","family":"Arulraj","sequence":"additional","affiliation":[{"name":"Centre for Plant Biotechnology, Department of Botany St. Xavier's College (Autonomous)  Palayamkottai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne Wincy Jacob","family":"Thomas","sequence":"additional","affiliation":[{"name":"Maria Research Foundation Alagar Nagar  Tirunelveli India"},{"name":"Department of Computer Science Sarah Tucker College (Autonomous), Perumalpuram  Tirunelveli India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6634-4207","authenticated-orcid":false,"given":"Henrique D. M.","family":"Coutinho","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry Regional University of Cariri  Crato Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,29]]},"reference":[{"key":"e_1_2_11_2_1","volume-title":"Working Together for Equity and Healthier Populations: Sustainable Multisectoral Collaboration Based on Health in all Policies Approaches","author":"World Health Organization","year":"2023"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbe.2018.01.004"},{"issue":"3","key":"e_1_2_11_4_1","first-page":"238","article-title":"Evaluation of Antioxidant Potential of Ethanolic Extracts of Selected Species of Selaginella","volume":"4","author":"Sivaraman A.","year":"2013","journal-title":"Indian Journal of Natural Products and Resources"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpc.2019.108664"},{"issue":"2","key":"e_1_2_11_6_1","first-page":"270","article-title":"Inhibitory Effect of Nano\u2010Formulated Extract of Passiflora Incarnata on Dalton's Lymphoma Ascites\u2010Bearing Swiss Albino Mice","volume":"17","author":"Deepika B.","year":"2025","journal-title":"Pharmacy"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202500626"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph13040066"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jddst.2024.106118"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2009.0852"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/2055773"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jams.2016.04.004"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42250\u2010021\u201000240\u20100"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/02726351.2016.1278292"},{"issue":"1","key":"e_1_2_11_15_1","first-page":"50","article-title":"A Review on Endemic Indian Resurrecting Herb Selaginella bryopteris (L.) 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