{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T18:12:34Z","timestamp":1788545554010,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Ipomoea carnea<\/jats:italic>\n                    , \u201cBush Morning Glory,\u201d belongs to the family\n                    <jats:italic>Convolvulaceae<\/jats:italic>\n                    and is cultivated in Egypt as an ornamental plant. Traditionally, its juice is used topically as an antiseptic for skin lesions. This study aims to identify the most effective solvent fraction for extracting antimicrobial compounds from\n                    <jats:italic>I. carnea<\/jats:italic>\n                    . Leaf and stem extracts were fractionated and tested for activity against\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    ,\n                    <jats:italic>Streptococcus mutans<\/jats:italic>\n                    ,\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    ,\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    ,\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    , and\n                    <jats:italic>Aspergillus niger<\/jats:italic>\n                    . Ultrahigh\u2010performance liquid chromatograms (UHPLC)\u2013HRMS analysis was conducted to profile metabolites of dichloromethane (DCM) extract of\n                    <jats:italic>I. carnea stem<\/jats:italic>\n                    . Isolated compounds from the same fraction were subsequently subjected to molecular docking with bacterial dihydrofolate reductase (DHFR) enzyme, and minimum inhibitory concentrations (MICs) were determined. DCM fraction of stem and butanol fractions of both leaf and stem demonstrated broad\u2010spectrum antimicrobial activity. UHPLC\u2013HRMS identified 21 metabolites, including coumarins, lactones, phenolic acid derivatives, and resin glycosides in stem DCM fraction. Three compounds\u2014ethyl caffeate, scopoletin, and umbelliferone\u2014were isolated. These compounds demonstrated significant binding to bacterial DHFR in molecular docking and showed confirmed antimicrobial activity via MIC testing. These encouraging results suggest that\n                    <jats:italic>I. carnea<\/jats:italic>\n                    has potential as a source of antimicrobial agents. However, further research is necessary to confirm its therapeutic applications against infectious diseases.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202502152","type":"journal-article","created":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T18:04:31Z","timestamp":1764266671000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Isolation and Characterization of Antimicrobial Agents From\n                    <i>Ipomoea carnea<\/i>\n                    via Bioactivity\u2010Guided Fractionation, Supported by\n                    <i>In Vitro<\/i>\n                    and\n                    <i>In Silico<\/i>\n                    Studies"],"prefix":"10.1002","volume":"23","author":[{"given":"Hamada","family":"Sadki Mohamed","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Beni\u2010Suef University  Beni\u2010Suef Egypt"},{"name":"Pharmacognosy Department College of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and Technology  6th of October City Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1411-1049","authenticated-orcid":false,"given":"Naglaa","family":"Afifi","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Beni\u2010Suef University  Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Sebak","sequence":"additional","affiliation":[{"name":"Microbiology and Immunology Department, Faculty of Pharmacy Beni\u2010Suef University  Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafik","family":"Mounir","sequence":"additional","affiliation":[{"name":"Pharmacognosy Department College of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and Technology  6th of October City Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina M. A. Abdel","family":"Ghany","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department College of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and Technology  6th of October City Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wesam E.","family":"Gawad","sequence":"additional","affiliation":[{"name":"Microbiology and Immunology Department College of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and Technology  6th of October City Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osama G.","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Pharmacognosy Department, Faculty of Pharmacy Cairo University  Cairo Egypt"},{"name":"Natural Products Discovery Core Life Sciences Institute University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9683-4250","authenticated-orcid":false,"given":"Rabab","family":"Mohammed","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Beni\u2010Suef University  Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,27]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118321492"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-13-8763-0"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.5040\/9798400664915"},{"key":"e_1_2_12_5_1","first-page":"463","article-title":"Medicinal Plants of Morning Glory: Convolvulaceae Juss. of Central India (Madhya Pradesh and Chhattishgarh)","volume":"2","author":"Sahu P. 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