{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T23:12:32Z","timestamp":1790723552842,"version":"4.1.0"},"reference-count":58,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T00:00:00Z","timestamp":1758931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T00:00:00Z","timestamp":1758931200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Myosotis amoena<\/jats:italic>\n                    Boiss. (Turkish name: \u201cHo\u015f boncukotu\u201d) is a perennial herbaceous plant that produces blue flowers during the spring. Phytochemicals, which are secondary metabolites distributed across plants, fungi, microorganisms, and even animal tissues, play crucial roles in health through their antioxidant and bioactive properties. Their antioxidant capacity largely arises from hydroxyl groups and aromatic ring structures that stabilize free radicals via electron delocalization. Enzyme inhibition is a therapeutic strategy in several diseases, including Alzheimer's disease (AD), where acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitors are employed to manage symptoms. This study investigated the phytochemical composition (via LC\u2013MS\/MS), total phenolic content, antioxidant capacity, and AChE\/BChE inhibitory effects of the ethanol extract of\n                    <jats:italic>M. amoena<\/jats:italic>\n                    using four in vitro methods. The CUPRAC assay demonstrated that\n                    <jats:italic>M. amoena<\/jats:italic>\n                    (1.070) exhibited higher antioxidant activity than Trolox (0.894), although its activity in FRAP, ABTS, and Fe\n                    <jats:sup>3+<\/jats:sup>\n                    reduction assays was lower compared to standard antioxidants. The extract contained 53.03\u00a0mg GAE\/g extract of total phenolics, with rosmarinic acid being the predominant compound (15.27\u00a0mg\/g). Furthermore, the extract strongly inhibited AChE and BChE, with IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 3.9 and 19.8\u00a0\u00b5g\/mL, respectively. These findings highlight, for the first time, the significant bioactive potential of\n                    <jats:italic>M. amoena<\/jats:italic>\n                    as a natural source of antioxidants and cholinesterase inhibitors, supporting its prospective use in the prevention or management of neurodegenerative conditions.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202502157","type":"journal-article","created":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T18:17:05Z","timestamp":1758997025000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Phytochemical Profile, Antioxidant Capacity, Acetylcholinesterase, and Butyrylcholinesterase Enzyme Inhibition Properties of\n                    <i>Myosotis amoena<\/i>\n                    Boiss"],"prefix":"10.1002","volume":"22","author":[{"given":"\u0130smail","family":"Yap\u0131c\u0131","sequence":"first","affiliation":[{"name":"Central Laboratory 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T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa Abdullah","family":"Yilmaz","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Faculty of Pharmacy Dicle University  Diyarbak\u0131r T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00fctfi","family":"Beh\u00e7et","sequence":"additional","affiliation":[{"name":"Molecular Biology and Genetics Department Sc\u0131ence and Art Faculty Bing\u00f6l University  Bing\u00f6l T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O\u011fuz","family":"Cak\u0131r","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Dicle University Science and Technology Research and Application Center  Diyarbakir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0130lhami","family":"G\u00fcl\u00e7in","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of 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