{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T18:51:04Z","timestamp":1788375064871,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T00:00:00Z","timestamp":1770854400000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Five\n                    <jats:italic>Siraitia grosvenorii<\/jats:italic>\n                    polysaccharides (SGPs), namely, SGP\u2010H, SGP\u2010A, SGP\u2010B, SGP\u2010E, and SGP\u2010U, were extracted from hot air\u2010dried fruits using hot water extraction (HWE), acid\u2010assisted extraction (ACAE), alkaline\u2010assisted extraction (ALAE), enzyme\u2010assisted extraction (EAE), and ultrasonic\u2010assisted extraction (UAE), respectively. The extraction yields of ACAE and ALAE were 1.65\u2010 and 1.59\u2010fold higher than that of traditional HWE, respectively. Monosaccharide composition analysis showed that SGP\u2010H, SGP\u2010A, SGP\u2010E, and SGP\u2010U were acidic heteropolysaccharides composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose with a notable discrepancy in the molar ratio, whereas SGP\u2010B exhibited the lowest uronic acid content and a unique monosaccharide profile dominated by glucose (76.62%). Comprehensive characterization confirmed significant differences in molecular weight, glycosidic linkage, and surface morphology among the five SGPs. Antioxidant assays indicated that SGP\u2010B exhibited the stronger ABTS and 2,2\u2010diphenyl\u20101\u2010picrylhydrazyl (DPPH) scavenging activity than the other SGPs, with EC\n                    <jats:sub>50<\/jats:sub>\n                    values of 416\u00a0\u00b1\u00a05.4 and 338\u00a0\u00b1\u00a014.8\u00a0\u00b5g\/mL, respectively. Meanwhile, immunomodulatory studies on RAW264.7 macrophages revealed that SGP\u2010B significantly enhanced phagocytosis by 29% and stimulated the secretion of immune factors. Therefore, SGP\u2010B, obtained via ALAE, shows great application potential in the development of antioxidants and immunomodulators, and further research deserves attention.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202502168","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T08:23:08Z","timestamp":1770884588000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Physicochemical Characterization and Bioactivities of Polysaccharides From\n                    <i>Siraitia grosvenorii<\/i>\n                    : Effects of Different Extraction Methods"],"prefix":"10.1002","volume":"23","author":[{"given":"Xiaohua","family":"Jiang","sequence":"first","affiliation":[{"name":"Guangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences  Guilin 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