{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T08:57:21Z","timestamp":1785488241917,"version":"3.56.0"},"reference-count":58,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T00:00:00Z","timestamp":1768348800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The essential oil of\n                    <jats:italic>Pilocarpus microphyllus<\/jats:italic>\n                    (jaborandi) (EOJ), a species traditionally recognized for its alkaloid\u2010based pharmacological properties, remains poorly investigated despite its richness in bioactive terpenes. In this study, the chemical profile of EOJ obtained from fresh and dried leaves was determined by gas chromatography\u2010mass spectrometry, revealing 24 constituents, predominantly \u03b3\u2010cadinene (23.6%) and\n                    <jats:italic>trans\u2010<\/jats:italic>\n                    caryophyllene (22.9%). Antifungal activity was observed against\n                    <jats:italic>Cryptococcus neoformans<\/jats:italic>\n                    (minimum inhibitory concentration: 149\u22122395\u00a0\u00b5g\/mL), while antileishmanial potential was confirmed against\n                    <jats:italic>Leishmania amazonensis<\/jats:italic>\n                    promastigotes (half\u2010maximal inhibitory concentration [IC\n                    <jats:sub>50<\/jats:sub>\n                    ]: 22.8\u201325.2\u00a0\u00b5g\/mL). EOJ also exhibited cytotoxic effects on HCT\u2010116 and PC\u20103 cell lines (IC\n                    <jats:sub>50<\/jats:sub>\n                    : 27.8\u201329.2\u00a0\u00b5g\/mL). In silico studies revealed strong binding affinities with therapeutic targets: \u03b3\u2010cadinene to Nectin\u20104 (\u0394G = \u22127.3\u00a0kcal\/mol) and\n                    <jats:italic>trans\u2010<\/jats:italic>\n                    caryophyllene to lanosterol 14\u03b1\u2010demethylase (\u0394G = \u22125.7\u00a0kcal\/mol). Absorption, distribution, metabolism, excretion, and toxicity predictions indicated favorable oral absorption and low genotoxicity. Altogether, EOJ demonstrates multitarget bioactivity, and its major constituents represent promising leads for antifungal, antileishmanial, and anticancer drug development.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202502175","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T14:26:06Z","timestamp":1768400766000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cytotoxic and Antileishmanial Potential of\n                    <i>Pilocarpus microphyllus<\/i>\n                    Essential Oil: In Vitro and In Silico Study"],"prefix":"10.1002","volume":"23","author":[{"given":"Juniel Cruz","family":"Silva","sequence":"first","affiliation":[{"name":"Biodiversity and Biotechnology Research Center BIOTEC Federal University of Delta do Parna\u00edba (UFDPAR)  Parna\u00edba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Gabriela Ara\u00fajo","family":"Mendes","sequence":"additional","affiliation":[{"name":"Advanced Study Group in Medical Mycology GEAMICOL Federal University of Delta do Parna\u00edba (UFDPAR)  Parna\u00edba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo S\u00e9rgio de Araujo","family":"Sousa","sequence":"additional","affiliation":[{"name":"Biodiversity and Biotechnology Research Center BIOTEC Federal University of Delta do Parna\u00edba (UFDPAR)  Parna\u00edba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alyne Rodrigues de Ara\u00fajo","family":"Nobre","sequence":"additional","affiliation":[{"name":"Biodiversity and Biotechnology Research Center BIOTEC Federal University of Delta do Parna\u00edba (UFDPAR)  Parna\u00edba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel Mu\u00e1lem de Moraes","family":"Alves","sequence":"additional","affiliation":[{"name":"Laboratory of Antileishmania Activity LAA) Medicinal Plants Research Center NPPM Federal University of Piau\u00ed UFPI)  Teresina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando A\u00e9cio de Amorim","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Laboratory of Antileishmania Activity LAA) Medicinal Plants Research Center NPPM Federal University of Piau\u00ed UFPI)  Teresina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiane Caroline","family":"Daboit","sequence":"additional","affiliation":[{"name":"Advanced Study Group in Medical Mycology GEAMICOL Federal University of Delta do Parna\u00edba (UFDPAR)  Parna\u00edba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 de Sousa Lima","family":"Neto","sequence":"additional","affiliation":[{"name":"Pharmacy Department Health Sciences Center Federal University of Piau\u00ed (UFPI)  Teresina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ygor Victor Ferreira","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Biodiversity and Biotechnology Research Center BIOTEC Federal University of Delta do Parna\u00edba (UFDPAR)  Parna\u00edba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ytallo da Costa","family":"Sousa","sequence":"additional","affiliation":[{"name":"Biodiversity and Biotechnology Research Center BIOTEC Federal University of Delta do Parna\u00edba (UFDPAR)  Parna\u00edba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edymila\u00eds da Silva","family":"Sousa","sequence":"additional","affiliation":[{"name":"Organic Geochemistry Laboratory Natural Sciences Center Federal University of Piau\u00ed (UFPI)  Teresina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Delano Barreto Marinho","family":"Filho","sequence":"additional","affiliation":[{"name":"Delta Cell Culture Laboratory LCCDelta) Federal University of Delta do Parna\u00edba (UFDPAR)  Parna\u00edba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana J\u00e9rsia","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Delta Cell Culture Laboratory LCCDelta) Federal University of Delta do Parna\u00edba (UFDPAR)  Parna\u00edba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Artur Silva","family":"Filho","sequence":"additional","affiliation":[{"name":"State University of Piau\u00ed  Parna\u00edba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sidney Gon\u00e7alo","family":"de Lima","sequence":"additional","affiliation":[{"name":"Advanced Study Group in Medical Mycology GEAMICOL Federal University of Delta do Parna\u00edba (UFDPAR)  Parna\u00edba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3422-2878","authenticated-orcid":false,"given":"Leiz Maria Costa","family":"V\u00e9ras","sequence":"additional","affiliation":[{"name":"Biodiversity and Biotechnology Research Center BIOTEC Federal University of Delta do Parna\u00edba (UFDPAR)  Parna\u00edba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph15060762"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijo.IJO_1172_24"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986712800167347"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.01.033"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1260448"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573\u2010020\u201000114\u2010z"},{"key":"e_1_2_10_8_1","first-page":"1","article-title":"Resist\u00eancia Antimicrobiana e Novos Antibi\u00f3ticos: 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