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This study aimed to isolate, characterize, and evaluate the bioactivity of four major flavonoids, identified as catechin, taxifolin, aromadendrin, and farrerol, from\n                    <jats:italic>C. terminalis<\/jats:italic>\n                    kino. Bioactive compounds were isolated through bioassay\u2010guided fractionation and preparative and semi\u2010preparative high\u2010performance liquid chromatography, and structurally identified using nuclear magnetic resonance spectroscopy and mass spectrometry. Antimicrobial activity was assessed against 19 bacterial strains using minimum inhibitory concentration (MIC) and agar well diffusion assays. Anti\u2010inflammatory effects were evaluated in lipopolysaccharide\u2010stimulated human keratinocyte cells via cytokine suppression (interleukin\u20106 [IL\u20106] and interleukin\u20108 [IL\u20108]) and enzyme inhibition assays targeting cyclooxygenase\u20101 (COX\u20101), cyclooxygenase\u20102 (COX\u20102), and 5\u2010lipoxygenase (5\u2010LOX). All four flavonoids exhibited significant antimicrobial and anti\u2010inflammatory activities. Farrerol showed the strongest antibacterial effect (MIC = 4\u2013512\u00a0\u00b5g\/mL), while catechin was most potent against\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    (MIC = 2\u00a0\u00b5g\/mL). Taxifolin and aromadendrin effectively suppressed IL\u20106 and IL\u20108 production (25\u201350\u00a0\u00b5g\/mL). Although all compounds inhibited COX\u20101 and COX\u20102, none exceeded the 5\u2010LOX inhibition by nordihydroguaiaretic acid. These findings support the traditional use of\n                    <jats:italic>C. terminalis<\/jats:italic>\n                    kino and its potential as a therapeutic source.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202502242","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T18:09:21Z","timestamp":1765994961000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Flavonoids From\n                    <i>Corymbia terminalis<\/i>\n                    Kino With Antimicrobial and Anti\u2010Inflammatory Activities"],"prefix":"10.1002","volume":"23","author":[{"given":"Marzieh","family":"Negahban","sequence":"first","affiliation":[{"name":"Innovative Medicines Group School of Clinical Sciences Faculty of Health Queensland University of Technology  Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Katavic","sequence":"additional","affiliation":[{"name":"Innovative Medicines Group School of Clinical Sciences Faculty of Health Queensland University of Technology  Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satish","family":"Dighe","sequence":"additional","affiliation":[{"name":"Innovative Medicines Group School of Clinical Sciences Faculty of Health Queensland University of Technology  Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Collet","sequence":"additional","affiliation":[{"name":"Innovative Medicines Group School of Clinical Sciences Faculty of Health Queensland University of Technology  Brisbane Australia"},{"name":"School of Biomedical Sciences Faculty of Health Queensland University of Technology  Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4826-8041","authenticated-orcid":false,"given":"Kamel","family":"Msaada","sequence":"additional","affiliation":[{"name":"Laboratory of Aromatic and Medicinal Plants Biotechnology Center in Borj C\u00e9dria Technopole  Hammam\u2010Lif Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trudi","family":"Collet","sequence":"additional","affiliation":[{"name":"Innovative Medicines Group School of Clinical Sciences Faculty of Health Queensland University of Technology  Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,17]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants14020206"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2024.106687"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906\u2010024\u201004437\u2010x"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics13080746"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.7751\/telopea19953017"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants12213686"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.14421\/biomedich.2024.131.147-157"},{"key":"e_1_2_12_9_1","first-page":"9317","article-title":"Optimizing Conditions for Microwave\u2010Assisted Solvent Extraction of Polar Compounds From Carissa carandas","volume":"89","author":"Kumar A.","year":"2024","journal-title":"J Agric Mach Eng"},{"key":"e_1_2_12_10_1","doi-asserted-by":"crossref","unstructured":"M.Negahban The Medicinal Effects of Two Australian Native Plants. 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