{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T08:45:00Z","timestamp":1775897100705,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Breast cancer persists as the most prevalent malignancy and principal contributor to cancer\u2010associated mortality among women worldwide, exhibiting marked disease heterogeneity and therapeutic resistance. Consequently, the development of novel therapeutic agents remains imperative. Ribociclib, a cyclin\u2010dependent kinase 4\/6 inhibitor, has emerged as a focus in adjuvant breast cancer research due to its mechanism of cell cycle arrest induction. Building upon this rationale, we designed and synthesized a novel Ribociclib derivative, RB\u2010BC, systematically investigating its anti\u2010neoplastic efficacy and molecular mechanisms in breast cancer through an integrated in vitro and in vivo experimental system. The compound's anti\u2010proliferative effects were quantitatively assessed via 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide assays, 5\u2010ethynyl\u20102\u2019\u2010deoxyuridine incorporation assays, and colony formation assays. Functional characterization through cell adhesion assays, scratch wound healing assays, and Transwell invasion assays demonstrated RB\u2010BC's dose\u2010dependent suppression of tumor migration and invasion. Mechanistic analyses, conducted via RNA sequencing and Western blotting, revealed that its anti\u2010tumor activity primarily stems from the activation of the p53 pathway, which induces caspase\u20103\u2010mediated apoptosis in breast cancer cells. Machine learning algorithms identified p53\u2010associated genetic signatures, and subsequent bioinformatics analyses elucidated their significant correlation with immune cell infiltration dynamics in the tumor microenvironment. Moreover, RB\u2010BC effectively suppressed tumor growth in vivo and significantly disrupted angiogenesis in the chick chorioallantoic membrane assay. In summary, the Ribociclib\u2010derived compound, RB\u2010BC, demonstrates compelling potential as a candidate therapeutic agent for breast cancer.<\/jats:p>","DOI":"10.1002\/cbdv.202502267","type":"journal-article","created":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T13:15:57Z","timestamp":1770124557000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["RB\u2010BC: A Novel Ribociclib Derivative Targeting p53\u2010Mediated Apoptosis and Inhibition of Tumor Growth in Breast Cancer"],"prefix":"10.1002","volume":"23","author":[{"given":"Boyu","family":"Zhang","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, College of Pharmacy Jiangsu Ocean University  Lianyungang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kadirya","family":"Asan","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Marine 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