{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T13:46:34Z","timestamp":1788270394991,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T00:00:00Z","timestamp":1764460800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T00:00:00Z","timestamp":1764460800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Pimpinella anisum<\/jats:italic>\n                    , an annual herb, has a long traditional history of being used as a treatment for various health\u2010related issues. The current study aimed to comprehensively understand how the methanolic extract of\n                    <jats:italic>P. anisum<\/jats:italic>\n                    seeds alleviates pain, reduces fever, and attenuates inflammation through molecular pathways and biochemical processes. Pain relief was evaluated using Eddy's hot plate test, tail immersion test, and acetic acid\u2010induced writhing models. Anti\u2010inflammatory study was conducted using three different methods; carrageenan induced paw edema, histamine\u2010induced paw edema and formalin\u2010induced paw edema models along with assessment of antipyretic activity. The extract demonstrated significant peripheral efficacy by reducing writhing occurrences. A comparable central pain elevation was also observed by prolongation of latency time in addition to substantial paw volume reduction in the carrageenan\u2010induced model, especially at the 300\u00a0mg\/kg dose. Histamine\u2010induced swelling significantly decreased, with the same dose exhibiting the highest inhibition percentage. In addition, complete inflammation reversal was observed by the fifth day of treatment, alongside a decrease in basal temperature, highlighting its antipyretic potential. GC\u2013MS analysis helped to identify key bioactive molecules, whereas docking analysis substantiated a significant analgesic and anti\u2010inflammatory effect. The extract's pain\u2010relieving effects were attributed to its interactions with COX\u20101 and glutamate receptors. These findings underscore\n                    <jats:italic>P. anisum<\/jats:italic>\n                    seeds potential as a natural remedy for pain, inflammation, and fever, suggesting avenues for further research and the development of innovative therapeutic approaches in herbal medicine.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202502270","type":"journal-article","created":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T23:49:46Z","timestamp":1764546586000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Valorization of Therapeutic Potential of\n                    <i>Pimpinella anisum<\/i>\n                    L. Seeds: A Study on Its Analgesic, Antipyretic, and Anti\u2010Inflammatory Effects"],"prefix":"10.1002","volume":"23","author":[{"given":"Aymun","family":"Jawed","sequence":"first","affiliation":[{"name":"Punjab University College of Pharmacy University of the Punjab Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5777-6112","authenticated-orcid":false,"given":"Muhammad Imran","family":"Tousif","sequence":"additional","affiliation":[{"name":"Department of Chemistry Division of Science and Technology University of Education  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rukshana","family":"Anwar","sequence":"additional","affiliation":[{"name":"Punjab University College of Pharmacy University of the Punjab Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Saleem","sequence":"additional","affiliation":[{"name":"Faculty of Chemical &amp; Biological Sciences Institute of Chemistry The Islamia University of Bahawalpur  Bahawalpur Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aamir","family":"Mushtaq","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences Government College University Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Humayun","family":"Riaz","sequence":"additional","affiliation":[{"name":"Rashid Latif College of Pharmacy Rashid Latif Medical Complex  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Umer","family":"Shaukat","sequence":"additional","affiliation":[{"name":"Rashid Latif College of Pharmacy Rashid Latif Medical Complex  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rabia","family":"liaqat","sequence":"additional","affiliation":[{"name":"Rashid Latif College of Pharmacy Rashid Latif Medical Complex  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syed Atif","family":"Raza","sequence":"additional","affiliation":[{"name":"Punjab University College of Pharmacy University of the Punjab Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gokhan","family":"Zengin","sequence":"additional","affiliation":[{"name":"Department of Biology Science Faculty Selcuk University  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,30]]},"reference":[{"key":"e_1_2_17_2_1","doi-asserted-by":"publisher","DOI":"10.5402\/2012\/510795"},{"key":"e_1_2_17_3_1","first-page":"46","article-title":"General Characteristics of Seeds of Some Anise (Pimpinella anisum L.) 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