{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T12:46:45Z","timestamp":1775998005102,"version":"3.50.1"},"reference-count":71,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:00:00Z","timestamp":1773360000000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Ferns and allies are underexplored sources of bioactives. This study evaluates the phytochemical composition and bioactivities of the ultrasound\u2010assisted ethanolic (CT\u2010UAE) extract of the dietary fern\n                    <jats:italic>Ceratopteris thalictroides<\/jats:italic>\n                    (L.) Brongn (\n                    <jats:italic>C. thalictroides<\/jats:italic>\n                    ). The extract contained notable levels of total phenolics (4.34 \u00b1 0.16\u00a0mg GAE\/g), flavonoids (3.74 \u00b1 0.08\u00a0mg QE\/g), and alkaloids (1.27 \u00b1 0.15\u00a0mg AE\/g). Volatile and semi\u2010volatile compounds were identified using GC\u2010MS\/MS, while LC\u2010MS\/MS profiling revealed non\u2010volatile and polar metabolites. The extract exhibited strong antibacterial activity, producing inhibition zones of 22 \u00b1 0.29\u00a0mm (\n                    <jats:italic>S. aureus<\/jats:italic>\n                    ), 22 \u00b1 0.89\u00a0mm (\n                    <jats:italic>B. subtilis<\/jats:italic>\n                    ), 24 \u00b1 0.44\u00a0mm (\n                    <jats:italic>E. coli<\/jats:italic>\n                    ), and 14 \u00b1 0.72\u00a0mm (\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    ), supported by MIC values of 60\u201390\u00a0\u00b5g\/mL. Anti\u2010inflammatory assays, including HRBC membrane stabilization and egg albumin denaturation, revealed IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 426.81 \u00b1 1.37\u00a0\u00b5g\/mL and 448.35 \u00b1 0.04\u00a0\u00b5g\/mL. The extract also showed cytotoxic activity against A431 carcinoma cells, with an IC\n                    <jats:sub>50<\/jats:sub>\n                    of 57.61\u00a0\u00b5g\/mL. Docking studies showed that quercetin has the strongest molecular interactions with FimH (\u20137.02\u00a0kcal\/mol), EGFR kinase (\u20137.13\u00a0kcal\/mol), and COX\u20102 (\u20138.91\u00a0kcal\/mol). These results indicate\n                    <jats:italic>C. thalictroides<\/jats:italic>\n                    as a promising source of antibacterial, anti\u2010inflammatory, and anticancer compounds for pharmaceutical and nutraceutical use.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202502352","type":"journal-article","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T09:07:22Z","timestamp":1773392842000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phytochemical Composition and Bioactivity of Ultrasound\u2010Assisted Ethanolic Extract of\n                    <i>Ceratopteris Thalictroides<\/i>\n                    (L.) Brongn: Integrated In Vitro and In Silico Evaluation of Antibacterial, Anticancer, and Anti\u2010Inflammatory Studies"],"prefix":"10.1002","volume":"23","author":[{"given":"Siyamol","family":"Thannikkappilly Johny","sequence":"first","affiliation":[{"name":"Department of Chemistry School of Advanced Sciences Vellore Institute of Technology  Vellore Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary","family":"Saral Antoneyraj","sequence":"additional","affiliation":[{"name":"Department of Chemistry School of Advanced Sciences Vellore Institute of Technology  Vellore Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.3308"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/5445291"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants13040523"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-818593-3.00003-8"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sciaf.2024.e02509"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13399-023-04726-4"},{"key":"e_1_2_9_8_1","first-page":"1","article-title":"Techniques for Extraction and Isolation of Natural Products: A Comprehensive Review","volume":"13","author":"Zhang Q. 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