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We investigated the neuroprotective effects of a novel polyherbal formulation (PHF) on CP\u2010induced neurotoxicity in SH\u2010SY5Y cells and its mechanism of action. PHF showed strong antioxidant action and mitigated CP\u2010induced SH\u2010SY5Y toxicity by significantly (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05\u20130.001) increasing the cell viability by 51.44%, 71.28%, and 98.43% at doses 250, 500, and 1000\u00a0\u00b5g\/mL, respectively. CP\u2010induced oxidative stress in SH\u2010SY5Y cells was associated with a significant (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.001) decline in DPPH (by 40.26%), ABTS (by 44.98%), and CUPRIC (by 68.95%) activities. Interestingly, PHF prevented lipid peroxidation, ROS and apoptosis, and enhanced MMP level and the antioxidant status in SH\u2010SY5Y cells. The phytocompounds detected in PHF showed strong binding affinity with CP\u2010activator enzymes (CYP2D6 and CYP2C19) and oxidative stress\u2010related markers (AChE and GST), which support the in vitro findings. PHF could be useful in the prevention\/treatment of chemotherapy\u2010associated neurodegenerative disorders.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202502363","type":"journal-article","created":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T18:41:12Z","timestamp":1765392072000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Neuroprotective Effects of a Novel Polyherbal Formulation Against Cyclophosphamide\u2010Induced Cytotoxicity in SH\u2010SY5Y Cells"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9865-4923","authenticated-orcid":false,"given":"Patrick Brice Defo","family":"Deeh","sequence":"first","affiliation":[{"name":"Department of Bio\u2010Health Convergence Kangwon National University  Chuncheon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeo","family":"Jinhee","sequence":"additional","affiliation":[{"name":"Jeongseon Country Agricultural Technology Center  Jeongseon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hye\u2010Yong","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Dental Hygiene, College of Health Science Kangwon National University  Chuncheon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su\u2010Ji","family":"Ryu","sequence":"additional","affiliation":[{"name":"Department of Bio\u2010Health Convergence Kangwon National University  Chuncheon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myeong\u2010Hyeon","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Bio\u2010Health Convergence Kangwon National University  Chuncheon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2024.118197"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology9050103"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines12020282"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568009614666140312145936"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1122629"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.10726"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2014.11.005"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726\u2010017\u20102527\u2010x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/wco.0b013e3283542fc2"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2024.2335299"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472\u20106882\u201013\u20104"},{"issue":"10","key":"e_1_2_9_13_1","first-page":"1403","article-title":"Antiinflammatory and Antioxidant Flavonoids From Helichrysum kraussii and H. odoratissimum Flowers","volume":"8","author":"Legoalea P. 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