{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T18:14:12Z","timestamp":1790100852920,"version":"4.0.1"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100006730","name":"Ministry of Education and Research, Romania","doi-asserted-by":"publisher","award":["PN\u2010IV\u2010P8\u20108.3\u2010ROMD\u20102023\u20100269"],"award-info":[{"award-number":["PN\u2010IV\u2010P8\u20108.3\u2010ROMD\u20102023\u20100269"]}],"id":[{"id":"10.13039\/501100006730","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The aim of this study was to investigate the ultrasound\u2010assisted extraction (UAE) of antioxidant flavonoids from sunflower (\n                    <jats:italic>Helianthus annuus<\/jats:italic>\n                    ) ray florets through the optimization of the parameters (temperature, extraction ratio, and solvent concentration) using a response surface methodology (RSM), then to validate the model conducting wet\u2010lab analyses, and finally, to characterize the optimized extracts for composition and in vitro cardioprotective potential. Within RSM, the parameters of UAE were optimized by maximization of the response variables yield of flavonoid extraction and Trolox equivalent antioxidant capacity. Two optimal solutions obtained in 73% ethanol, at 30:1 (v\/w) extraction ratio and 80\u00b0C (extract 1\u2032) and in 77% ethanol, at 12:1 (v\/w) extraction ratio and 40\u00b0C (extract 2\u2032) were validated. HPLC analysis revealed that rutin and myricetin were the main flavonoids in both optimized extracts and slightly higher amounts were quantified in extract 2\u2032. In addition, extract 2\u2032 presented higher free radical scavenging activity and angiotensin\u2010converting enzyme (ACE) inhibition than extract 1\u2032. Both extracts were cytocompatible in an endothelial cell culture, in a wide range of concentrations (31.25\u2013500\u00a0\u00b5g\/mL) and stimulated the cell metabolism, after 24\u00a0h of cultivation. Optimized extract 2\u2032 containing flavonoids from sunflower ray florets with antioxidant properties and cardioprotective potential was also a solution of interest for the bioeconomy, due to less consuming extraction conditions. This extract could be recommended for further testing as a valuable ingredient of novel cardioprotective nutraceuticals.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202502370","type":"journal-article","created":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T18:01:00Z","timestamp":1764266460000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Optimization of Antioxidant Flavonoids Extraction From\n                    <i>Helianthus annuus<\/i>\n                    L. Ray Florets for Cardioprotective Nutraceuticals"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0625-3650","authenticated-orcid":false,"given":"Oana","family":"Craciunescu","sequence":"first","affiliation":[{"name":"National Institute of R&amp;D for Biological Sciences  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Mihai","sequence":"additional","affiliation":[{"name":"National Institute of R&amp;D for Biological Sciences  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Gaspar\u2010Pintiliescu","sequence":"additional","affiliation":[{"name":"National Institute of R&amp;D for Biological Sciences  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana\u2010Maria","family":"Seciu\u2010Grama","sequence":"additional","affiliation":[{"name":"National Institute of R&amp;D for Biological Sciences  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Duca","sequence":"additional","affiliation":[{"name":"Moldova State University  Chisinau Republic of Moldova"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steliana","family":"Clapco","sequence":"additional","affiliation":[{"name":"Moldova State University  Chisinau Republic of Moldova"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandru","family":"Ciocarlan","sequence":"additional","affiliation":[{"name":"Moldova State University  Chisinau Republic of Moldova"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena Iulia","family":"Oprita","sequence":"additional","affiliation":[{"name":"National Institute of R&amp;D for Biological Sciences  Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.31080\/ASPS.2021.05.0752"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biombioe.2021.106265"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/horticulturae11020206"},{"issue":"2","key":"e_1_2_9_5_1","first-page":"1","article-title":"In Vivo and In Vitro Studies of a Polar Extract of Helianthus annuus (Sunflower) Seeds in Treatment of Napkin Dermatitis","volume":"24","author":"Aziz F. 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