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To this end, in vivo tests were performed on adult zebrafish (Danio rerio) and in silico molecular docking analyses were conducted. Acute toxicity (96\u00a0h) was evaluated with doses of 4, 20, and 40\u00a0mg\/kg (\n                    <jats:italic>i.p<\/jats:italic>\n                    .). Motor activity and anxiety levels induced by the different doses were evaluated using open field and light\/dark tests, while the anticonvulsant potential was tested by induction with pentylenetetrazol (PTZ). The GABAergic neuromodulation was also investigated using the antagonist flumazenil and the molecular interaction with the GABA\n                    <jats:sub>A<\/jats:sub>\n                    receptor and carbonic anhydrase II (CAII). The results demonstrated that arjunolic acid is not toxic at the tested doses, but it does cause significant motor alterations, like those caused by diazepam. The compound exhibited an anxiolytic effect and increased the latency to the onset of convulsive seizures. These effects were reversed by flumazenil, confirming mediation by the GABAergic system. These results corroborate the in silico study, which demonstrated a possible allosteric effect of arjunolic acid on the diazepam binding region of the GABA\n                    <jats:sub>A<\/jats:sub>\n                    receptor and on the active site of CAII. However, arjunolic acid is pharmacologically relevant to the central nervous system and may serve as a basis for the development of new therapeutic agents.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202502547","type":"journal-article","created":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T11:05:38Z","timestamp":1773486338000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["GABAergic Modulation and Neurobehavioral Effects of Arjunolic Acid, a Pentacyclic Triterpene Isolated From\n                    <i>Combretum Mellifluum<\/i>\n                    Eichler: In Vivo and Molecular Docking Evaluation"],"prefix":"10.1002","volume":"23","author":[{"given":"Yanna Julie da Silva","family":"Freitas","sequence":"first","affiliation":[{"name":"Graduate Program in Natural Sciences State University of Cear\u00e1  Fortaleza Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00e9ssica Bezerra","family":"Maciel","sequence":"additional","affiliation":[{"name":"Graduate Program in Natural Sciences State University of Cear\u00e1  Fortaleza Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Eduarda Uchoa","family":"Bezerra","sequence":"additional","affiliation":[{"name":"Graduate Program in Natural Sciences State University of Cear\u00e1  Fortaleza Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor Moreira","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Graduate Program in Natural Sciences State University of Cear\u00e1  Fortaleza Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone Alves Serafim","family":"Rocha","sequence":"additional","affiliation":[{"name":"Department of Organic and Inorganic Chemistry Federal University of Cear\u00e1  Fortaleza Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ot\u00edlia Loiola","family":"Pessoa","sequence":"additional","affiliation":[{"name":"Department of Organic and Inorganic Chemistry Federal University of Cear\u00e1  Fortaleza Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcia Machado","family":"Marinho","sequence":"additional","affiliation":[{"name":"Graduate Program in Natural Sciences State University of Cear\u00e1  Fortaleza Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuel Silva","family":"Marinho","sequence":"additional","affiliation":[{"name":"Graduate Program in Natural Sciences State University of Cear\u00e1  Fortaleza Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e9ia Rocha de Sousa","family":"Feitosa","sequence":"additional","affiliation":[{"name":"Graduate Program in Natural Sciences State University of Cear\u00e1  Fortaleza Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane Eire Silva Alencar","family":"de Menezes","sequence":"additional","affiliation":[{"name":"Graduate Program in Natural Sciences State University of Cear\u00e1  Fortaleza Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreia Ferreira de Castro","family":"Gomes","sequence":"additional","affiliation":[{"name":"Center For Molecular and Environmental Biology School of Sciences Department of Biology University of Minho  Braga Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5527-164X","authenticated-orcid":false,"given":"H\u00e9lcio Silva","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Graduate Program in Natural Sciences State University of Cear\u00e1  Fortaleza Cear\u00e1 Brazil"},{"name":"Center For Exact Sciences and Technology State University of Vale do Acara\u00fa  Sobral Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/198053146952"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpsychires.2019.09.007"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yebeh.2021.107881"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2023.2167116"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.34119\/bjhrv7n1\u2010086"},{"key":"e_1_2_9_7_1","first-page":"1","volume":"38","author":"Uzai F. 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