{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T16:00:40Z","timestamp":1785081640624,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:00:00Z","timestamp":1778630400000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The rise of\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    (Kp) as an MDR pathogen has become a pressing global health challenge, contributing to UTIs and Pneumonia. A major factor underlying its persistence and antibiotic resistance is its inherent ability to form protective biofilm matrices. This study explores a natural approach to combat Kp biofilms by leveraging the antibacterial potential of plant\u2010derived essential oils. Among bioactive compounds, 2\u2010Phenyl ethyl methyl ether, a constituent of kewda oil, appears as a viable option. Our investigation revealed that 2\u2010PEME exhibits significant antibacterial activity against Kp with a MIC of 50\u00a0mM, and the clinical isolate was further confirmed by morphological and molecular characterization. Notably, at a sub\u2010MIC concentration of 25\u00a0mM, 2\u2010PEME disrupted biofilm formation, reducing biofilm biomass by 61.17% \u00b1 3.75% and 59.30% \u00b1 6.24% in Kp and MTCC\u2010109, respectively. These findings were further corroborated through microscopic analysis, providing strong evidence of 2\u2010PEME's biofilm\u2010inhibiting potential. To elucidate the mechanism of action, molecular docking studies were performed, which predicted strong binding affinities between 2\u2010PEME and key biofilm\u2010associated and drug\u2010resistant proteins in Kp. Cell viability assays conducted on HEK\u2010293, 3T3L1, and L929 cell lines demonstrated no cytotoxic effects, confirming their viability as a safe alternative. Computational pharmacokinetic analysis further supported its drug\u2010like properties.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202502552","type":"journal-article","created":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T18:23:26Z","timestamp":1778696606000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antibacterial and Antibiofilm Efficacy of 2\u2010Phenyl Ethyl Methyl Ether Against Multidrug\u2010Resistant\n                    <i>Klebsiella pneumoniae<\/i>"],"prefix":"10.1002","volume":"23","author":[{"given":"Monika","family":"Mishra","sequence":"first","affiliation":[{"name":"Centre of Excellence in Natural Products and Therapeutics, Department of Biotechnology and Bioinformatics Sambalpur University  Sambalpur Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subhaswaraj","family":"Pattnaik","sequence":"additional","affiliation":[{"name":"Centre of Excellence in Natural Products and Therapeutics, Department of Biotechnology and Bioinformatics Sambalpur University  Sambalpur Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pratyush","family":"Pragyandipta","sequence":"additional","affiliation":[{"name":"Centre of Excellence in Natural Products and Therapeutics, Department of Biotechnology and Bioinformatics Sambalpur University  Sambalpur Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eeshara","family":"Naik","sequence":"additional","affiliation":[{"name":"Centre of Excellence in Natural Products and Therapeutics, Department of Biotechnology and Bioinformatics Sambalpur University  Sambalpur Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dibyadarshini","family":"Sahu","sequence":"additional","affiliation":[{"name":"Centre of Excellence in Natural Products and Therapeutics, Department of Biotechnology and Bioinformatics Sambalpur University  Sambalpur Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aparajita","family":"Priyadarshini","sequence":"additional","affiliation":[{"name":"Department of Food Science Technology and Nutrition Sambalpur University  Sambalpur Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7044-2427","authenticated-orcid":false,"given":"Pradeep Kumar","family":"Naik","sequence":"additional","affiliation":[{"name":"Centre of Excellence in Natural Products and Therapeutics, Department of Biotechnology and Bioinformatics Sambalpur University  Sambalpur Odisha 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