{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T08:01:40Z","timestamp":1785484900673,"version":"3.56.0"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T00:00:00Z","timestamp":1768694400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Chagas disease, a neglected tropical disease, affects millions of individuals in developing countries. Due to the limitations of current therapeutic options, the search for new bioactive compounds is crucial. Brazilian red propolis, a resinous bee product from northeastern Brazil, is recognized for its diverse chemical constituents and biological, including antiparasitic, activities. This study evaluated the in vitro activity of a polyprenylated benzophenone\u2010enriched Brazilian red propolis extract (\n                    <jats:bold>SEBz<\/jats:bold>\n                    ) and its constituents, the mixture of isomers guttiferone E and xanthochymol (\n                    <jats:bold>GUT\/XAN<\/jats:bold>\n                    ), and oblongifolin B (\n                    <jats:bold>OBL<\/jats:bold>\n                    ), against\n                    <jats:italic>Trypanosoma cruzi<\/jats:italic>\n                    (Y strain) trypomastigote and amastigote forms. Cytotoxicity and hemolysis assays assess safety.\n                    <jats:bold>SEBz<\/jats:bold>\n                    exhibited antiparasitic activity, with 50% effective concentration (EC\n                    <jats:sub>50<\/jats:sub>\n                    ) values of 17.97\u00a0\u00b5g mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    (trypomastigotes) and 6.83\u00a0\u00b5g mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    (amastigotes) after 48\u00a0h, while demonstrating moderate cytotoxicity on the C2C12 myoblast cell line (50% cytotoxicity concentration [CC\n                    <jats:sub>50<\/jats:sub>\n                    ] 42.80\u00a0\u00b5g mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    at 48\u00a0h). Both\n                    <jats:bold>GUT\/XAN<\/jats:bold>\n                    and\n                    <jats:bold>OBL<\/jats:bold>\n                    showed trypanocidal effects (EC\n                    <jats:sub>50<\/jats:sub>\n                    \u2264 5\u00a0\u00b5M against trypomastigotes;\n                    <jats:bold>GUT\/XAN<\/jats:bold>\n                    EC\n                    <jats:sub>50<\/jats:sub>\n                    7.91\u00a0\u00b5M on amastigotes), with moderate cytotoxicity (CC\n                    <jats:sub>50<\/jats:sub>\n                    &lt; 20\u00a0\u00b5M). Neither the extract nor the compounds induced significant hemolysis. Ultrastructural analysis of treated parasites revealed nuclear deformation and vacuolation. These findings support the potential of Brazilian red propolis benzophenones as promising leads for Chagas disease drug development.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202502682","type":"journal-article","created":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T09:33:00Z","timestamp":1768728780000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Trypanocidal Effects of Polyprenylated Benzophenone Enriched Brazilian Red Propolis Extract and Its Isolated Constituent"],"prefix":"10.1002","volume":"23","author":[{"given":"Nicoli Dias","family":"Oliveira","sequence":"first","affiliation":[{"name":"Center For Research in Sciences and Technology University of Franca. Avenida Dr. Armando Salles of Oliveira  Franca Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Cintra","family":"Pagotti","sequence":"additional","affiliation":[{"name":"Center For Research in Sciences and Technology University of Franca. Avenida Dr. Armando Salles of Oliveira  Franca Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Antonio de Lima","family":"Paula","sequence":"additional","affiliation":[{"name":"Center For Research in Sciences and Technology University of Franca. Avenida Dr. Armando Salles of Oliveira  Franca Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Zaramello","family":"Paix\u00e3o","sequence":"additional","affiliation":[{"name":"Center For Research in Sciences and Technology University of Franca. Avenida Dr. Armando Salles of Oliveira  Franca Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daiane","family":"Albino dos Santos","sequence":"additional","affiliation":[{"name":"Center For Research in Sciences and Technology University of Franca. Avenida Dr. Armando Salles of Oliveira  Franca Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata Faleiros","family":"de Freitas","sequence":"additional","affiliation":[{"name":"Center For Research in Sciences and Technology University of Franca. Avenida Dr. Armando Salles of Oliveira  Franca Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9rgio Ricardo","family":"Ambr\u00f3sio","sequence":"additional","affiliation":[{"name":"Center For Research in Sciences and Technology University of Franca. Avenida Dr. Armando Salles of Oliveira  Franca Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Cassio Sola","family":"Veneziani","sequence":"additional","affiliation":[{"name":"Center For Research in Sciences and Technology University of Franca. Avenida Dr. Armando Salles of Oliveira  Franca Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jairo Kenupp","family":"Bastos","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences of Ribeir\u00e3o Preto University of S\u00e3o Paulo. Av. Do Caf\u00e9  Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2959-6752","authenticated-orcid":false,"given":"Lizandra Guidi","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"Center For Research in Sciences and Technology University of Franca. Avenida Dr. Armando Salles of Oliveira  Franca Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","unstructured":"World Health Organization (WHO).Chagas Disease (American trypanosomiasis) Fact Sheet. Available at Chagas disease (also known as American trypanosomiasis)\u00a0(Accessed November 14 2025).","DOI":"10.5826\/dpc.1402a81"},{"key":"e_1_2_10_3_1","unstructured":"Drugs for Neglected Diseases initiative (DNDi).Chagas Disease: Symptoms Transmission and Current Treatments. 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