{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T07:00:13Z","timestamp":1775890813664,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T00:00:00Z","timestamp":1770249600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Justicia betonica<\/jats:italic>\n                    L. (Acanthaceae) is a traditionally used medicinal plant with limited evidence on its anticancer mechanisms. This study integrates phytochemical profiling, molecular docking, and in vitro\/in vivo evaluations to elucidate its anticancer potential. Gas chromatography\u2013mass spectrometry (GC\u2013MS) and liquid chromatography\u2013mass spectrometry (LC\u2013MS) analyses identified a diverse phytochemical repertoire, including retronecine, maritimetin, hematopodin, mangiferin 6\u2032\u2010gallate, and quercetin glycosides, many reported here for the first time in\n                    <jats:italic>J. betonica<\/jats:italic>\n                    L. In silico docking revealed high binding affinities of these constituents toward multiple oncogenic targets\u2014CDK\u20102, CDK\u20106, B\u2010cell lymphoma 2 (Bcl\u20102), vascular endothelial growth factor receptor\u20102 (VEGFR\u20102), and insulin\u2010like growth Factor 1 receptor (IGF\u20101R)\u2014suggesting multi\u2010target inhibitory potential. The ethanolic extract of\n                    <jats:italic>J. betonica<\/jats:italic>\n                    L. exhibited selective cytotoxicity against MCF\u20107, A549, and HT29 cell lines, with IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 42.11\u00a0\u00b1\u00a01.64, 35.39\u00a0\u00b1\u00a01.02, and 29.66\u00a0\u00b1\u00a01.34\u00a0\u00b5g\/mL, respectively, while sparing normal human umbilical vein endothelial cells (HUVECs). Acute toxicity studies confirmed safety up to 2000\u00a0mg\/kg. In the Ehrlich ascites carcinoma (EAC) model, oral administration of EEJB (400\u00a0mg\/kg) significantly reduced tumor volume, packed cell volume, and viable cell count, extended mean survival time, and ameliorated tumor\u2010induced hematological and hepatic alterations. These findings position\n                    <jats:italic>J. betonica<\/jats:italic>\n                    L. as a promising plant\u2010derived, multi\u2010target anticancer candidate, meriting further isolation of active principles and mechanistic exploration.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202502688","type":"journal-article","created":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T21:28:56Z","timestamp":1770326936000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multi\u2010Target Anticancer Activity of\n                    <i>Justicia betonica<\/i>\n                    L.: Phytochemical Profiling, In Silico Screening, and Preclinical Evaluation"],"prefix":"10.1002","volume":"23","author":[{"given":"Sai Prasanna","family":"Rasamalla","sequence":"first","affiliation":[{"name":"Drug Discovery Laboratory School of Pharmacy GITAM (Deemed to be) University Hyderabad Telangana 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