{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T07:00:07Z","timestamp":1775890807136,"version":"3.50.1"},"reference-count":54,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T00:00:00Z","timestamp":1766102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T00:00:00Z","timestamp":1766102400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Fermented\n                    <jats:italic>Brassica<\/jats:italic>\n                    \u2010based foods generate diverse bioactive metabolites through microbial transformation. This study examined two traditionally fermented mustard leaf products from Northeast India\u2014\n                    <jats:italic>\u1e6cam\u2010um<\/jats:italic>\n                    (TUM) and\n                    <jats:italic>\u1e6cam\u2010um tengkhang<\/jats:italic>\n                    (TUTM)\u2014for their chemical composition and anti\u2010inflammatory potential. Methanolic extracts of TUM and TUTM were analyzed using ultra\u2010high\u2010performance liquid chromatography coupled with Q\u2010Orbitrap high\u2010resolution mass spectrometry (UHPLC\u2010Q\u2010Orbitrap HRMS). Antioxidant and anti\u2010inflammatory activities were evaluated through cell\u2010free assays and in LPS\u2010stimulated RAW 264.7 macrophages. Gene and protein expression of inflammatory mediators were determined by quantitative real\u2010time PCR (qRT\u2010PCR) and Western blotting. Molecular docking was performed between the major identified metabolite, 11\u2010(methylsulfinyl) undecyl glucosinolate, and murine tumor necrosis factor\u2010alpha (TNF\u2010\u03b1) and IL\u20106. Metabolomic profiling revealed glucosinolates as key constituents, dominated by 11\u2010(methylsulfinyl) undecyl glucosinolate. Both extracts showed high reactive oxygen species (ROS)\u2010scavenging activity and upregulated SOD2 expression. They also suppressed nitric oxide production and reduced TNF\u2010\u03b1, IL\u20106, and inducible nitric oxide synthase (\n                    <jats:italic>iNOS<\/jats:italic>\n                    ) expression. Western blot results indicated inhibition of phosphorylated p38 and SAPK\/JNK, suggesting modulation of the mitogen\u2010activated protein kinase (MAPK) pathway, whereas nuclear factor\u2010kappa B (NF\u2010\u03baB) remained largely unaffected. Docking results showed stronger binding of the major glucosinolate to TNF\u2010\u03b1 than epigallocatechin gallate and comparable affinity for IL\u20106. Fermented mustard extracts demonstrated significant antioxidant and anti\u2010inflammatory effects, primarily through modulation of MAPK signaling rather than NF\u2010\u03baB suppression. These findings highlight their potential as functional, redox\u2010active foods with specific molecular targets.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202502765","type":"journal-article","created":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T17:06:09Z","timestamp":1766163969000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metabolomic Profiling and Mechanistic Insights Into the Antioxidant and Anti\u2010Inflammatory Effects of Traditional Fermented Mustard From Mizoram"],"prefix":"10.1002","volume":"23","author":[{"given":"Vanlalhruaii","family":"Famhawite","sequence":"first","affiliation":[{"name":"Centre for Pre\u2010Clinical Studies CSIR\u2010North East Institute of Science and Technology (NEIST)  Jorhat Assam India"},{"name":"AcSIR\u2010Academy of Scientific and Innovative Research  Ghaziabad Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deep Jyoti","family":"Das","sequence":"additional","affiliation":[{"name":"Centre for Pre\u2010Clinical Studies CSIR\u2010North East Institute of Science and Technology (NEIST)  Jorhat Assam India"},{"name":"AcSIR\u2010Academy of Scientific and Innovative Research  Ghaziabad Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. 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