{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T20:14:21Z","timestamp":1783368861152,"version":"3.54.6"},"reference-count":76,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Use of 10 different combinations of aldehydes and amines to carry out the Mannich reaction on 8\u2010hydroxyquinoline generated 11 compounds including Mannich bases and imines, 6 of which are new while 5 are known. Their structures were characterized by mass spectroscopy,\n                    <jats:sup>1<\/jats:sup>\n                    HNMR,\n                    <jats:sup>13<\/jats:sup>\n                    CNMR including 2D, IR, and EI analysis. Upon screening for their antimicrobial activities, oxidative burst (of whole blood phagocytes, isolated neutrophils and macrophages), on T\u2010cell proliferation, antioxidant and cytotoxic activities, compound\n                    <jats:bold>4<\/jats:bold>\n                    (7\u2010[\u03b1\u2010(2\u2033,5\u2033\u2010dichloroanilinobenzyl]\u20108\u2010hydroxyquinoline) a new Mannich base, was found to possess antimicrobial property, immunomodulating potential on chemiluminescence assays, suppressed T\u2010cells and exhibited no cytotoxicity. This novel compound upon in vivo histopathological examination on BALB\/c mice showed unaffected kidneys at any of the tested concentration (1.3, 6.67, 33.33, and 166.67\u00a0mg\/kg), while at different concentrations were found to be cardio\u2010toxic and hepatotoxic. Furthermore, molecular docking simulations determined the probable binding modes of these compounds to their targets.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202502792","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T19:31:16Z","timestamp":1781551876000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synthesis of Known and New 8\u2010Hydroxyquinoline Mannich Bases and Determination of Their Significant Biological Activities"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-2579-6930","authenticated-orcid":false,"given":"Farheen","family":"Ansari","sequence":"first","affiliation":[{"name":"Department of Microbiology University of Karachi  Karachi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaheen","family":"Faizi","sequence":"additional","affiliation":[{"name":"HEJ Research Institute of Chemistry International Centre For Chemical and Biological Sciences University of Karachi  Karachi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Humaira","family":"Siddiqi","sequence":"additional","affiliation":[{"name":"HEJ Research Institute of Chemistry International Centre For Chemical and Biological Sciences University of Karachi  Karachi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omer M.","family":"Abdalla","sequence":"additional","affiliation":[{"name":"Dr. Panjwani Centre For Molecular Medicine and Drug Research International Centre for Chemical and Biological Sciences University of Karachi  Karachi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samina N.","family":"Mukry","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Karachi  Karachi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rubeena","family":"Saleem","sequence":"additional","affiliation":[{"name":"Department of Chemistry Jinnah University for Women  Karachi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad A.","family":"Mesaik","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Faculty of Medicine University of Tabuk  Tabuk Saudi Arabia"},{"name":"Molecular and Infectious Diseases Research Unit (RDIA) Deanship of Scientific Research University of Tabuk  Tabuk Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurul","family":"Kabir","sequence":"additional","affiliation":[{"name":"Dr. Panjwani Centre For Molecular Medicine and Drug Research International Centre for Chemical and Biological Sciences University of Karachi  Karachi Pakistan"},{"name":"Institute of Biological Sciences Faculty of Science University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aqeel","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Karachi  Karachi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hina","family":"Qaiser","sequence":"additional","affiliation":[{"name":"Dr. Panjwani Centre For Molecular Medicine and Drug Research International Centre for Chemical and Biological Sciences University of Karachi  Karachi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaheer","family":"Ul\u2010Haq","sequence":"additional","affiliation":[{"name":"Dr. Panjwani Centre For Molecular Medicine and Drug Research International Centre for Chemical and Biological Sciences University of Karachi  Karachi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shakeel A.","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Karachi  Karachi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,15]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Physical Properties, Chemical Properties of Oxine and Its Derivatives","author":"Hollingshead R. 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