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This study investigated the anti\u2010ZIKV potential of an ethanolic extract from\n                    <jats:italic>Terminalia glabrescens<\/jats:italic>\n                    leaves. Dereplication of the extract by UPLC\u2013ESI\u2013MS\/MS identified 27 phenolic compounds, while GC\u2013MS analysis of the dichloromethane (DCM) fraction revealed carboxylic acids, esters, an alcohol, a triterpene, and hydrocarbons. The extract (30\u00a0\u00b5g\/mL) reduced viral load by 2.6 log in Vero CCL\u201081 cells and inhibited viral replication in SH\u2010SY5Y cells (CC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a0130.2\u00a0\u00b1\u00a027.5\u00a0\u00b5g\/mL; EC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a020.4\u00a0\u00b1\u00a010.2\u00a0\u00b5g\/mL; SI\u00a0=\u00a06.4). Bioguided fractionation localized the antiviral activity to the DCM fraction, which reduced viral load by 5.1 log in Vero CCL\u201081 and 4.0 log in SH\u2010SY5Y cells. Chromatographic fractionation of DCM fraction afforded glutinol, \u03b2\u2010sitosterol, and a mixture of \u03b1\/\u03b2\u2010amyrin. Among these, \u03b2\u2010sitosterol exhibited the most significant activity against ZIKV in SH\u2010SY5Y cells (CC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0&gt;\u00a0300\u00a0\u00b5M; EC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a071.3\u00a0\u00b1\u00a07.1\u00a0\u00b5M; SI\u00a0&gt;\u00a04.2). A 2D similarity analysis based on structural fingerprints revealed that \u03b2\u2010sitosterol is structurally distinct from known anti\u2010ZIKV compounds in the ChEMBL database, underscoring its novelty and potential as a lead scaffold for antiviral drug development. These findings identify \u03b2\u2010sitosterol as a promising candidate for the development of anti\u2010ZIKV drugs.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202502860","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T15:17:09Z","timestamp":1768403829000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Bioactivity\u2010Guided Isolation of \u03b2\u2010Sitosterol From\n                    <i>Terminalia glabrescens<\/i>\n                    as a Potent Anti\u2010Zika Virus Agent"],"prefix":"10.1002","volume":"23","author":[{"given":"Ros\u00e2ngela Santos","family":"Pereira","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Products, Faculty of Pharmacy Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priscilla Rodrigues Valadares","family":"Campana","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Products, Faculty of Pharmacy Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivian Vasconcelos","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Institute of Biological Sciences Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vin\u00edcius Gon\u00e7alves","family":"Maltarollo","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Products, Faculty of Pharmacy Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Maia","family":"de P\u00e1dua","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Products, Faculty of Pharmacy Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Hugo Sousa","family":"de Gomes","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Products, Faculty of Pharmacy Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grasiely Farias","family":"de Sousa","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Institute of Exact Sciences Universidade Federal De Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Dias","family":"de Souza Filho","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Institute of Exact Sciences Universidade Federal De Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele da Gl\u00f3ria","family":"de Souza","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Institute of Biological Sciences Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro Martins","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Institute of Biological Sciences Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9468-376X","authenticated-orcid":false,"given":"Fern\u00e3o Castro","family":"Braga","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Products, Faculty of Pharmacy Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2112.151167"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(16)00562\u20106"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMsr1604338"},{"key":"e_1_2_8_5_1","unstructured":"Brasil.Minist\u00e9rio da Sa\u00fade.Informe Semanal: Dengue e Outras Arboviroses. 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