{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:51:42Z","timestamp":1791237102270,"version":"4.1.0"},"reference-count":65,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Natural products rich in natural ingredients play a supporting role in the treatment of many diseases. This study evaluated the biological activities of\n                    <jats:italic>Citrus limon<\/jats:italic>\n                    (L.) Osbeck extracts obtained under optimized conditions using two techniques: response surface methodology (RSM) and artificial neural network\u2010genetic algorithm (ANN\u2010GA). Optimization focused on temperature, extraction time, and ethanol\u2010water ratio. The ANN\u2010GA model predicted optimal conditions as 51.629\u00b0C, 6.502 h, and 41.585% ethanol, while RSM suggested 53.219\u00b0C, 13.461 h, and 41.535% ethanol. Antioxidant properties were assessed via DPPH, FRAP, and TAS\/TOS assays. Anticholinesterase activity was measured through AChE and BChE inhibition, and antiproliferative effects were tested on A549 lung cancer cells. LC\u2010MS\/MS analysis identified major phenolic compounds, including gallic acid, caffeic acid, quercetin, naringenin, and kaempferol. ANN\u2010GA extracts exhibited higher antioxidant, anticholinesterase, and antiproliferative activities than those obtained by RSM. These effects may be linked to the higher phenolic content detected in ANN\u2010GA extracts. The findings highlight\n                    <jats:italic>C. limon<\/jats:italic>\n                    as a promising source of natural antioxidants and bioactives with potential therapeutic applications, particularly in neurodegenerative diseases and cancer. Further studies, including in vivo tests, are recommended to explore the full therapeutic potential of these extracts.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202502875","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T12:44:29Z","timestamp":1772973869000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Optimization of Dried\n                    <i>Citrus limon<\/i>\n                    Extracts Using Statistical Methods and Evaluation of Their In Vitro Antioxidant, Anticholinesterase, and Cytotoxic Activities"],"prefix":"10.1002","volume":"23","author":[{"given":"Orhan","family":"Unal","sequence":"first","affiliation":[{"name":"Faculty of Science Department of Biology Akdeniz University Antalya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oguzhan","family":"Ko\u00e7er","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Services, Vocational School of Health Services Osmaniye Korkut Ata University  Osmaniye T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imran","family":"Uysal","sequence":"additional","affiliation":[{"name":"Department of Food Processing, Bah\u00e7e Vocational School Osmaniye Korkut Ata University  Osmaniye T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ay\u015fenur","family":"G\u00fcrgen","sequence":"additional","affiliation":[{"name":"Faculty of Engineering and Natural Science, Department of Industrial Engineering Osmaniye Korkut Ata University  Osmaniye T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7223-2220","authenticated-orcid":false,"given":"Mustafa","family":"Sevindik","sequence":"additional","affiliation":[{"name":"Faculty of Engineering and Natural Science Department of Biology Osmaniye Korkut Ata University  Osmaniye 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