{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T16:04:00Z","timestamp":1785341040168,"version":"3.55.0"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:00:00Z","timestamp":1777248000000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    For centuries, medicinal plants have played a vital role in healthcare.\n                    <jats:italic>Phyllanthus amarus<\/jats:italic>\n                    is one such plant traditionally used to manage diabetes. This study combined computational modeling and laboratory experiments to evaluate the antidiabetic and antioxidant properties of its methanol extract (MEPa). MEPa exhibited notable in vitro antidiabetic activity by inhibiting key enzymes involved in glucose metabolism, with IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 10.2 \u00b5g\/mL (\u03b1\u2010amylase) and 12.5 \u00b5g\/mL (\u03b1\u2010glucosidase), compared to acarbose (6.4 and 8.5 \u00b5g\/mL). In RIN\u20105F insulinoma cells, MEPa improved cell viability (87.6% \u00b1 2.6%), reduced reactive oxygen species (43.6% \u00b1 4.2%), enhanced insulin secretion (155.6% \u00b1 11.2%), and inhibited TNF\u2010\u03b1 release (55.1% \u00b1 5.5%; IC\n                    <jats:sub>50<\/jats:sub>\n                    = 21.3 \u00b1 2.8 \u00b5g\/mL). The extract also showed high total polyphenol (153.2 \u00b1 3.6\u00a0mg GAE\/g) and flavonoid contents (114.5 \u00b1 2.8\u00a0mg QE\/g), contributing to strong antioxidant activity, including DPPH scavenging (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 31.4 \u00b1 0.7 \u00b5g\/mL), FRAP (EC\n                    <jats:sub>50<\/jats:sub>\n                    = 37.2 \u00b1 5.8 \u00b5g\/mL), and lipid peroxidation inhibition (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 5.8 \u00b1 0.8 \u00b5g\/mL). Computational analysis identified DL\u2010\u03b1\u2010tocopherol and \u03b3\u2010sitosterol as key bioactive compounds with strong binding affinities to \u03b1\u2010amylase and Keap1, respectively, surpassing standard inhibitors. These findings highlight MEPa as a promising natural source for antidiabetic and antioxidant drug development.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202503009","type":"journal-article","created":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T10:06:31Z","timestamp":1777284391000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unlocking the Antidiabetic and Antioxidant Potentials of\n                    <i>Phyllanthus amarus<\/i>\n                    Using In Vitro and In Silico Approaches"],"prefix":"10.1002","volume":"23","author":[{"given":"Success","family":"Ikoya","sequence":"first","affiliation":[{"name":"Drug Discovery and Translational Research Unit Department of Medical Biochemistry Delta State University  Abraka Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8930-437X","authenticated-orcid":false,"given":"Joy Onyewonuwa","family":"Acha","sequence":"additional","affiliation":[{"name":"Drug Discovery and Translational Research Unit Department of Medical Biochemistry Delta State University  Abraka Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa Ilobekemen","family":"Ekakitie","sequence":"additional","affiliation":[{"name":"Drug Discovery and Translational Research Unit Department of Medical Biochemistry Delta State University  Abraka Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel Nduka","family":"Enudinisu","sequence":"additional","affiliation":[{"name":"Drug Discovery and Translational Research Unit Department of Medical Biochemistry Delta State University  Abraka Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pearl Ufuoma","family":"Egbowawa","sequence":"additional","affiliation":[{"name":"Drug Discovery and Translational Research Unit Department of Medical Biochemistry Delta State University  Abraka Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Alex\u2010Igoru","sequence":"additional","affiliation":[{"name":"Drug Discovery and Translational Research Unit Department of Medical Biochemistry Delta State University  Abraka Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ejiro","family":"Obrirhe","sequence":"additional","affiliation":[{"name":"Drug Discovery and Translational Research Unit Department of Medical Biochemistry Delta State University  Abraka Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith Oghenevwogaga","family":"Okan","sequence":"additional","affiliation":[{"name":"Drug Discovery and Translational Research Unit Department of Medical Biochemistry Delta State University  Abraka Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Innocent","family":"Onyesom","sequence":"additional","affiliation":[{"name":"Drug Discovery and Translational Research Unit Department of Medical Biochemistry Delta State University  Abraka Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,27]]},"reference":[{"issue":"5","key":"e_1_2_8_2_1","first-page":"547","article-title":"Diabetes Mellitus and Oxidative Stress\u2010A Concise Review","volume":"24","author":"Ullah A.","year":"2015","journal-title":"Saudi Pharmacognosy Journal"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28207156"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.20454\/jeaas.2016.1087"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-021-02924-8"},{"key":"e_1_2_8_6_1","article-title":"Alpha\u2010Glucosidase and Alpha\u2010Amylase Inhibitory Effects of Antidiabetic Drugs and Their Side Effects: A Review","volume":"25","author":"Kandhare A. 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