{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:43:01Z","timestamp":1791646981450,"version":"4.3.4"},"reference-count":61,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100018786","name":"Al-Zaytoonah University of Jordan","doi-asserted-by":"publisher","award":["28\/13\/2024\u20132025"],"award-info":[{"award-number":["28\/13\/2024\u20132025"]}],"id":[{"id":"10.13039\/501100018786","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018786","name":"Al-Zaytoonah University of Jordan","doi-asserted-by":"publisher","award":["27\/06\/2024\u20102025"],"award-info":[{"award-number":["27\/06\/2024\u20102025"]}],"id":[{"id":"10.13039\/501100018786","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Wounds are a common cause of skin infections, and natural products are widely explored for their therapeutic potential. This study investigated the wound\u2010healing activity of\n                    <jats:italic>Salvia greggii<\/jats:italic>\n                    A.Gray (Lamiaceae). Aerial parts were collected and extracted using different solvents. Total phenolic and flavonoid contents were determined by Folin\u2013Ciocalteu and colorimetric assays, respectively, while antioxidant activity was assessed using the DPPH method. Extracts were screened for cell viability, and the most active fractions were evaluated using a scratch assay in human gingival fibroblasts. An in vivo excision wound model in rats was used to assess wound contraction over 14 days. The methanol extract showed the highest phenolic and flavonoid contents, whereas the ethyl acetate extract exhibited the strongest antioxidant activity. LC\u2013MS analysis of the methanol fraction identified compounds including benzoic, rosmarinic, and ferulic acids. Methanol and chloroform extracts enhanced cell viability, and the methanol extract achieved over 90% wound closure in vitro. In vivo, methanol\u2010treated wounds demonstrated 97.85% contraction and improved tissue regeneration. Additionally, the methanol extract significantly reduced mRNA expression of pro\u2010inflammatory cytokines (Tnf\u2010\u03b1, Il\u20101\u03b2, and Il\u20106). These findings suggest that\n                    <jats:italic>S. greggii<\/jats:italic>\n                    , particularly its methanol extract, promotes wound healing through antioxidant and anti\u2010inflammatory mechanisms.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202503045","type":"journal-article","created":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T07:05:09Z","timestamp":1775027109000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Therapeutic Potential of\n                    <i>Salvia greggii<\/i>\n                    Extracts in Promoting Wound Healing: Insights From Scratch Assay, Wound Model, and Molecular Analysis"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3800-1220","authenticated-orcid":false,"given":"Alaa M.","family":"Hammad","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Faculty of Pharmacy Al\u2010Zaytoonah University of Jordan  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baraa","family":"Al\u2010hajjaj","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Pharmacy Al\u2010Zaytoonah University of Jordan  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nour","family":"Aboalhaija","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Pharmacy Al\u2010Zaytoonah University of Jordan  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heba","family":"Syaj","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Pharmacy Al\u2010Zaytoonah University of Jordan  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma","family":"Afifi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy The University of Jordan  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osama H.","family":"Abusara","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Pharmacy Al\u2010Zaytoonah University of Jordan  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,1]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules16097672"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/22311866.2021.1906320"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.31925\/farmacia.2024.1.18"},{"issue":"2","key":"e_1_2_11_5_1","first-page":"261","article-title":"In Vitro and in Vivo Evaluation of the Wound Healing Potential of the Extracts of Schinus Molle L.(Anacardiaceae) Grown in Jordan","volume":"83","author":"Aboalhaija N.","year":"2021","journal-title":"Indian Journal of Pharmaceutical Sciences"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40495-022-00296-7"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/1354750X.2022.2085800"},{"issue":"1","key":"e_1_2_11_8_1","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1002\/(SICI)1099-1573(199902)13:1<50::AID-PTR368>3.0.CO;2-V","article-title":"Asiaticoside\u2010Induced Elevation of Antioxidant Levels in Healing Wounds","volume":"13","author":"Shukla A.","year":"1999","journal-title":"Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics14112288"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1515\/JBCPP.2009.20.1.73"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/cimb47120972"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2023.154824"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781003243472-20"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781003346142-17"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000039397"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23123169"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3897\/vz.62.e31404"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.flora.2017.10.004"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.20902\/IJPTR.2017.10326"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2017.05.053"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-024-03075-x"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0324930"},{"issue":"1","key":"e_1_2_11_23_1","first-page":"2885","article-title":"Physiological and Histological Effects of Formalin on Kidney of Rats and the Protective Role of Vitamin C","volume":"22","author":"Al\u2010Khatawi G. M.","year":"2022","journal-title":"Biochemical & Cellular Archives"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.2174\/1871530321666201223104622"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2025.115600"},{"key":"e_1_2_11_27_1","first-page":"3","article-title":"Rat Pro\u2010Inflammatory Cytokine and Cytokine Related mRNA Quantification by Real\u2010Time Polymerase Chain Reaction Using SYBR Green","volume":"5","author":"Peinnequin A.","year":"2004","journal-title":"BMC Immunology [Electronic Resource]"},{"issue":"7","key":"e_1_2_11_28_1","first-page":"53","article-title":"Effect of Extraction Solvents on Phenolic, Flavonoid and Antioxidant Activities of Three Nigerian Medicinal Plants","volume":"9","author":"Anokwuru C.","year":"2011","journal-title":"Nature and Science"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules15107313"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2010.12.026"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfda.2013.11.001"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules14062167"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants6040042"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.vh.2022.10.008"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep16147"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202500181"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-04195-x"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hermed.2023.100659"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf030681s"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2024.2335666"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2013.06.015"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42452-024-06023-9"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods13111740"},{"issue":"1","key":"e_1_2_11_44_1","first-page":"161","article-title":"Assessment of DPPH Free Radical Scavenging Activity of Some Medicinal Plants","volume":"1","author":"Asadujjaman M.","year":"2013","journal-title":"Pharmacologyonline"},{"issue":"1","key":"e_1_2_11_45_1","first-page":"25","article-title":"The Chemical and Biological Role of Pimaranes and Labdanes From Mexican Salvia Species","volume":"50","author":"Ortiz\u2010Mendozaa N.","year":"2023","journal-title":"Revista Latinoamericana de Qu\u00edmica"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2022.115066"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12051079"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcis.2024.12.138"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2009.06.007"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/972028"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00067.2017"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aam7928"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/imcb.12236"},{"issue":"3","key":"e_1_2_11_54_1","first-page":"159","article-title":"Chronic Wounds: Evaluation and Management","volume":"101","author":"Bowers S.","year":"2020","journal-title":"American Family Physician"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.200223"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1395479"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(07)57004-X"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/2684108"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18051068"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/iwj.70742"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11050700"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines8050101"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202503045","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.202503045","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202503045","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T15:49:12Z","timestamp":1785340152000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202503045"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":61,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/cbdv.202503045"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202503045","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"2025-09-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e03045"}}