{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T08:01:42Z","timestamp":1785484902921,"version":"3.56.0"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T00:00:00Z","timestamp":1767657600000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The genus\n                    <jats:italic>Stachys<\/jats:italic>\n                    (Lamiaceae) comprises numerous species recognized for their ethnopharmacological importance and rich chemical profiles. In this study, we investigated the chemical composition and biocompatibility of both wild\u2010growing Cretan samples and asexually propagated samples of\n                    <jats:italic>Stachys cretica<\/jats:italic>\n                    L. subsp.\n                    <jats:italic>cretica<\/jats:italic>\n                    that were ex situ cultivated in the same region (Crete). The methanol (MeOH) extracts and infusions were analyzed using high\u2010performance liquid chromatography\u2010photodiode array detection coupled with mass spectrometry (HPLC\u2010PDA\u2010MS) and proton nuclear magnetic resonance (\n                    <jats:sup>1<\/jats:sup>\n                    H\u2010NMR) spectroscopy. A total of 23 compounds were identified in the analyzed samples by HPLC\u2010PDA\u2010MS, classified as flavonoids, phenylethanoid glycosides, and phenolic acids. The NMR screening confirmed the presence of chlorogenic acid, acteoside, lavandulifolioside, and leucosceptoside A in the MeOH extract of wild\u2010growing\n                    <jats:italic>S. cretica<\/jats:italic>\n                    subsp.\n                    <jats:italic>cretica<\/jats:italic>\n                    . Methanol extracts exhibited higher chemical diversity compared to infusions, particularly in flavonoids. Moreover, biocompatibility is crucial for most biomedical and pharmaceutical applications, ensuring that drugs, drug delivery systems, and cosmetics avoid possible side effects or cytotoxicity. Thus, normal human embryonic kidney cells (HEK293) were cultivated and cell viability (%) was estimated. This confirmed that\n                    <jats:italic>S. cretica<\/jats:italic>\n                    subsp.\n                    <jats:italic>cretica<\/jats:italic>\n                    extracts are biocompatible. The limited phytochemical variation observed between wild\u2010growing and cultivated samples suggests that cultivation within the species\u2019 native range may preserve its chemical composition.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202503054","type":"journal-article","created":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T03:41:02Z","timestamp":1767757262000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring the Chemical Fingerprint and In Vitro Biocompatibility of Wild\u2010Growing and Ex Situ Cultivated\n                    <i>Stachys cretica<\/i>\n                    subsp.\n                    <i>cretica<\/i>\n                    in the Same Region (Crete, Greece)"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4313-9657","authenticated-orcid":false,"given":"Ekaterina\u2010Michaela","family":"Tomou","sequence":"first","affiliation":[{"name":"Department of Pharmacy Section of Pharmacognosy and Chemistry of Natural Products School of Health Sciences National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Anagnostou","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Section of Pharmacognosy and Chemistry of Natural Products School of Health Sciences National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco Cesare","family":"Battisti","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Section of Pharmacognosy and Chemistry of Natural Products School of Health Sciences National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia","family":"Karioti","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacognosy School of Pharmacy Aristotle University of Thessaloniki University Campus  Thessaloniki Greece"},{"name":"Department of Pharmacy University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikos","family":"Krigas","sequence":"additional","affiliation":[{"name":"Institute of Plant Breeding and Genetic Resources Hellenic Agricultural Organization Demeter (ELGO\u2010Dimitra)  Thermi Greece"},{"name":"Department of Viticulture Floriculture &amp; Plant Protection Institute of Olive Tree Subtropical Crops and Viticulture Hellenic Agricultural Organization\u2010Demeter (ELGO\u2010Dimitra)  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nefeli","family":"Lagopati","sequence":"additional","affiliation":[{"name":"Department 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