{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T21:11:07Z","timestamp":1789506667348,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T00:00:00Z","timestamp":1773619200000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004242","name":"Princess Nourah Bint Abdulrahman University","doi-asserted-by":"publisher","award":["PNURSP2026R33"],"award-info":[{"award-number":["PNURSP2026R33"]}],"id":[{"id":"10.13039\/501100004242","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Stress burdens the activities of individuals and negatively impacts their overall well\u2010being, highlighting the urgent need to explore effective remedies. Since the discovery of the first antimalarial drug from\n                    <jats:italic>Artemisia annua<\/jats:italic>\n                    , this plant has been recognized for its remarkable pharmacological activities, such as antibacterial, anticancer, anti\u2010obesity, and antidiabetic, among others. Herein, we investigated the phytochemical profiling and anti\u2010stress potential of\n                    <jats:italic>A. annua<\/jats:italic>\n                    using a mouse model. First, the crude extracts of\n                    <jats:italic>A. annua (<\/jats:italic>\n                    ArAn\u2010Crd) were subjected to HPLC and GC\u2010MS analyses, which revealed the presence of complex phytochemicals. According to results of the preliminary pharmacological assessment and acute toxicity study, doses of 125 and 250\u00a0mg\/kg body weight (b.w.) were selected to be effective doses for pharmacological activities. Subsequently, the anti\u2010stress and antidepressant properties were assessed using chemical\u2010induced stress, swimming endurance, and restraint stress models. In the chemical\u2010induced stress model, ArAn\u2010Crd at 250\u00a0mg reduced writhing by 71.14%, comparable to diazepam with 76.11% activity. In the swimming endurance test, the 250\u00a0mg dose significantly decreased immobility time to 81.65 \u00b1 2.98\u00a0s on Day 28, compared to 207.67 \u00b1 3.33\u00a0s in the control group. The restraint stress model revealed that ArAn\u2010Crd reduced blood glucose, triglycerides, and cholesterol levels, while also modulating oxidative stress biomarkers, such as TBARS, GSH, and catalase. Overall, the results of this study demonstrate that\n                    <jats:italic>A. annua<\/jats:italic>\n                    exhibits good anti\u2010stress and antidepressant activities due to its rich phytochemical profile, and possibly these phytochemicals mitigate the stress by an antioxidant mechanism.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202503075","type":"journal-article","created":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T16:54:46Z","timestamp":1773680086000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Antistress and Antidepressant Potentials of\n                    <i>Artemisia annua<\/i>\n                    Extract in a Mice Model"],"prefix":"10.1002","volume":"23","author":[{"given":"Subhan","family":"Ullah","sequence":"first","affiliation":[{"name":"Department of Biochemistry University of Malakand  Chakdara Khyber Pakhtunkhwa Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumaira","family":"Naz","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Malakand  Chakdara Khyber Pakhtunkhwa Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4528-8517","authenticated-orcid":false,"given":"Muhammad","family":"Zahoor","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Malakand  Chakdara Khyber Pakhtunkhwa Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adnan","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Malakand  Chakdara Khyber Pakhtunkhwa Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Esa","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Department of Pharmaceutical Technology, Drug Delivery System Excellence Center Prince of Songkla University  Hat Yai Songkhla Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syed Wadood Ali","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Malakand  Chakdara Khyber Pakhtunkhwa Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal","family":"Alotaibi","sequence":"additional","affiliation":[{"name":"Department of Basic Science, College of Medicine Princess Nourah Bint Abdulrahman University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,16]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781032672236-1"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21144986"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10886\u2010014\u20100384\u20106"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chmed.2018.11.002"},{"issue":"1","key":"e_1_2_12_6_1","first-page":"54","article-title":"Aqueous and Alcoholic Extracts of Artemisia annua L. 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