{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:49:33Z","timestamp":1792183773103,"version":"4.4.0"},"reference-count":86,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The increasing incidence of diabetes and antimicrobial resistance highlights the urgent need for plant\u2010based therapeutics with multifunctional health benefits. This study evaluated the phytochemical composition and biological activities of lotus (\n                    <jats:italic>Nelumbo nucifera<\/jats:italic>\n                    ) seed extracts obtained via Soxhlet extraction (SL) and ultrasonic\u2010assisted extraction (UL) using methanol as the extraction solvent. The UL extract showed higher yield and greater total phenolic (107.92\u00a0mg GAE\/g) and flavonoid (104.89\u00a0mg QE\/g) contents compared with SL. UHPLC\u2010DAD analysis identified\n                    <jats:italic>p\u2010coumaric acid<\/jats:italic>\n                    (5.60\u00a0mg\/g) and chlorogenic acid (4.27\u00a0mg\/g) as the dominant constituents in UL. The UL extract exhibited superior antioxidant activity DPPH\u2022 (IC\n                    <jats:sub>50<\/jats:sub>\n                    : 17.51\u00a0\u00b5g\/mL), although lower than the reference standards ascorbic acid (IC\n                    <jats:sub>50<\/jats:sub>\n                    : 12.74\u00a0\u00b5g\/mL) and BHT (IC\n                    <jats:sub>50<\/jats:sub>\n                    : 10.74\u00a0\u00b5g\/mL). It also demonstrated notable enzyme inhibition against \u03b1\u2010amylase (IC\n                    <jats:sub>50<\/jats:sub>\n                    : 12.54\u00a0\u00b5g\/mL), comparable but slightly weaker than the standard acarbose (IC\n                    <jats:sub>50<\/jats:sub>\n                    : 10.73\u00a0\u00b5g\/mL), and \u03b1\u2010glucosidase (IC\n                    <jats:sub>50<\/jats:sub>\n                    : 49.21\u00a0\u00b5g\/mL), relative to acarbose (IC\n                    <jats:sub>50<\/jats:sub>\n                    : 43.14\u00a0\u00b5g\/mL). In contrast, the SL extract exhibited stronger antimicrobial activity, particularly against\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (MIC: 1.0\u00a0mg\/mL). Molecular docking analysis identified rutin (\u22129.4\u00a0kcal\/mol) as the most potent \u03b1\u2010amylase inhibitor, supported by favorable ADMET properties and predicted high gastrointestinal absorption. These findings suggest that lotus seed extracts, particularly those obtained via ultrasonic extraction, represent promising candidates for functional food development and phytopharmaceutical applications in oxidative stress and diabetes management.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202503151","type":"journal-article","created":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T11:04:11Z","timestamp":1780657451000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Integrated\n                    <i>In Vitro<\/i>\n                    and\n                    <i>In Silico<\/i>\n                    Evaluation of Lotus (\n                    <i>Nelumbo nucifera<\/i>\n                    ) Seed Extracts for Antioxidant, Antimicrobial, and Antidiabetic Potentials"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0269-1302","authenticated-orcid":false,"given":"Shiwa","family":"Chaubey","sequence":"first","affiliation":[{"name":"Phytochemistry Division CSIR\u2010National Botanical Research Institute  Lucknow Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4603-0216","authenticated-orcid":false,"given":"Khuraijam Jibankumar","family":"Singh","sequence":"additional","affiliation":[{"name":"Botanical Garden Division CSIR\u2010National Botanical Research Institute  Lucknow Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8772-6159","authenticated-orcid":false,"given":"Manjoosha","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Phytochemistry Division CSIR\u2010National Botanical Research Institute  Lucknow Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,5]]},"reference":[{"key":"e_1_2_17_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20184567"},{"key":"e_1_2_17_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-443-22106-4.00022-X"},{"key":"e_1_2_17_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomimetics9070390"},{"key":"e_1_2_17_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/healthcare11131946"},{"key":"e_1_2_17_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-09686-5"},{"key":"e_1_2_17_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ddc3010011"},{"key":"e_1_2_17_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aninu.2024.01.012"},{"key":"e_1_2_17_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-031-44746-4_26"},{"key":"e_1_2_17_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology14060695"},{"key":"e_1_2_17_11_1","doi-asserted-by":"publisher","DOI":"10.29261\/pakvetj\/2025.123"},{"key":"e_1_2_17_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/blsf2024040015"},{"key":"e_1_2_17_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-97-9865-0_2"},{"key":"e_1_2_17_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu15224723"},{"key":"e_1_2_17_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods14101656"},{"key":"e_1_2_17_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.seppur.2023.124147"},{"key":"e_1_2_17_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods13152335"},{"key":"e_1_2_17_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules21070930"},{"key":"e_1_2_17_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/10942912.2022.2074030"},{"key":"e_1_2_17_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nexres.2025.100897"},{"key":"e_1_2_17_21_1","first-page":"12","article-title":"Phytocomponents and Antioxidant Activity in the Methanol Extract of Seed lotus (Nelumbo nucifera Gaernt.) 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