{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T08:01:44Z","timestamp":1785484904880,"version":"3.56.0"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"publisher","award":["2022CFB429"],"award-info":[{"award-number":["2022CFB429"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The shell of\n                    <jats:italic>Euryale ferox Salisb<\/jats:italic>\n                    ., rich in polyphenols, has antitumor potential. We isolated and identified polyphenols from the shell and evaluated their in vitro antitumor activity. Compounds were extracted with 70% ethanol under ultrasound and purified by fractional extraction and silica gel chromatography, yielding three substances. Liquid chromatography\u2013mass spectrometry (LC\u2010MS) and nuclear magnetic resonance confirmed the three compounds as ellagic acid, corilagin, and geraniin. High\u2010performance LC quantification showed corilagin and geraniin concentrations of 66.23\u00a0and 58.41\u00a0mg\/g. In vitro experiments on cells showed the ethanol extract had strong inhibitory effects on cell proliferation (IC\n                    <jats:sub>50<\/jats:sub>\n                    : 68.56 \u00b5g\/mL for HeLa, 78.09 \u00b5g\/mL for A2780), and cell proliferation, migration, and invasion were regulated by the three compounds, exhibiting time\u2010 and dose\u2010dependent characteristics. Corilagin showed the strongest inhibitory effect on HeLa cell proliferation (74.61% at 48\u00a0h) and A2780 cell invasion (69.86%). Geraniin inhibited HeLa cell invasion (63.33%) and A2780 cell invasion (61.64%). This study determined the chemical structures of polyphenolic compounds from\n                    <jats:italic>Euryale ferox Salisb<\/jats:italic>\n                    . shell and their impact on tumor cell proliferation, migration, and invasion, offering evidence for developing antitumor drugs and insights for modernizing traditional Chinese medicinal resources.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202503188","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T10:40:56Z","timestamp":1767868856000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Isolation, Identification, and in Vitro Antitumor Activity of Polyphenols From\n                    <i>Euryale ferox Salisb<\/i>\n                    . Shell"],"prefix":"10.1002","volume":"23","author":[{"given":"Xinru","family":"Jian","sequence":"first","affiliation":[{"name":"School of Life Science and Technology Wuhan Polytechnic University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiantian","family":"Shan","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology Wuhan Polytechnic University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhan","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology Wuhan Polytechnic University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology Wuhan Polytechnic University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haotian","family":"Ge","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology Wuhan Polytechnic University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longjie","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology Wuhan Polytechnic University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxun","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology Wuhan Polytechnic University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9378-1496","authenticated-orcid":false,"given":"Limei","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology Wuhan Polytechnic University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28114399"},{"key":"e_1_2_10_3_1","first-page":"787","article-title":"Antioxidant Activity of Euryale ferox Seed Shell Extract and Its Therapeutic Effects on Oral Ulcer in Rats","volume":"44","author":"Wang Q.","year":"2024","journal-title":"Nan Fang Yi Ke Da Xue Xue Bao"},{"key":"e_1_2_10_4_1","first-page":"104","article-title":"Antioxidant and Antimicrobial Activities of the Polysaccharides From Euryale ferox","volume":"40","author":"Xiangli L.","year":"2014","journal-title":"Food and Fermentation Industries"},{"key":"e_1_2_10_5_1","first-page":"151","article-title":"Antioxidative and Antibacterial Activities of Euryale ferox Salisb. Shell Extracts and Its Compositions Analysis","volume":"36","author":"Xiangli L.","year":"2015","journal-title":"Science and Technology of Food Industry"},{"key":"e_1_2_10_6_1","unstructured":"W.Sun \u201cStudy on the Effect of Anti\u2010oxidation Function and Hypoglycemic by Euryale ferox Salisb. Shells Extract \u201d(master's thesis Hefei University of Technology 2012)."},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2019.00406"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/iub.1731"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2019.01.044"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.2478\/intox-2018-0027"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.5650\/jos.ess19018"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2023.0185"},{"key":"e_1_2_10_13_1","first-page":"859","article-title":"Hypoglycemic Effect of Triterpenoid\u2010rich Extracts From Euryale ferox Shell on Normal and Streptozotocin\u2010diabetic Mice","volume":"27","author":"Yuan H. B.","year":"2014","journal-title":"Pakistan Journal of Pharmaceutical Sciences"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2025.139909"},{"key":"e_1_2_10_15_1","article-title":"The Effect and Mechanism of Corilagin From Euryale ferox Salisb. Shell on LPS\u2010Induced Inflammation in Raw264.7 Cells","volume":"12","author":"Wu M.","year":"2023","journal-title":"Foods"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfpp.12332"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.133669"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules30030722"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0286274"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules15031453"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2014.10.007"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/1099-1573(200008)14:5<371::AID-PTR625>3.0.CO;2-F"},{"key":"e_1_2_10_23_1","first-page":"888","article-title":"Research on the Anti\u2010cancer Effect and Mechanism of Ellagic Acid","volume":"44","author":"Tang R.","year":"2016","journal-title":"Modern Medical Journal"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1177\/11779322251316864"},{"key":"e_1_2_10_25_1","first-page":"1","article-title":"Effect of Corilagin on Proliferation and Apoptosis of human Cervical Cancer HeLa Cells","volume":"9","author":"Xu J.","year":"2018","journal-title":"Heilongjiang Science"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/8868508"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2025.156576"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.16274"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2021.08.076"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e38951"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2017.07.007"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24183399"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2024.101906"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21422"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202503188","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.202503188","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202503188","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T07:55:32Z","timestamp":1785484532000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202503188"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1002\/cbdv.202503188"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202503188","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-09-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e03188"}}