{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T12:45:48Z","timestamp":1775997948382,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:00:00Z","timestamp":1773878400000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In this study, bioassay\u2010guided fractionation of the ethanolic extract from\n                    <jats:italic>Eremanthus crotonoides<\/jats:italic>\n                    leaves enabled the isolation of two bioactive sesquiterpene lactones, centratherin and goyazensolide, which were evaluated for their antimycobacterial and anti\u2010inflammatory properties. The extract exhibited MIC\n                    <jats:sub>50<\/jats:sub>\n                    values of 42.0 \u00b1 0.1 and 39.0 \u00b1 0.1\u00a0\u00b5g\/mL against\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    H\n                    <jats:sub>37<\/jats:sub>\n                    Rv and the hypervirulent M299 strain, respectively, along with notable NO inhibitory activity. Fractionation by high\u2010speed countercurrent chromatography (HSCCC), followed by purification also employing HSCCC, yielded centratherin and goyazensolide, representing the first report of their isolation from\n                    <jats:italic>E. crotonoides<\/jats:italic>\n                    using this technique. These compounds showed potent activity against H\n                    <jats:sub>37<\/jats:sub>\n                    Rv (MIC\n                    <jats:sub>50<\/jats:sub>\n                    = 1.5 \u00b1 0.1 and 2.5 \u00b1 0.1\u00a0\u00b5g\/mL, respectively) but markedly reduced activity against M299 (MIC\n                    <jats:sub>50<\/jats:sub>\n                    = 92.7 \u00b1 0.1 and 90.6 \u00b1 0.1\u00a0\u00b5g\/mL). Both compounds also exhibited IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 0.45 \u00b1 0.1 and 0.34 \u00b1 0.1\u00a0\u00b5g\/mL for nitric oxide inhibition, with low cytotoxicity. Collectively, these findings provide the first evidence of the antimycobacterial potential of these compounds against virulent\n                    <jats:italic>M. tuberculosis<\/jats:italic>\n                    strains and underscore their promise as potential drug candidates.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202503250","type":"journal-article","created":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T07:25:08Z","timestamp":1773905108000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antimycobacterial and Nitric Oxide Production Inhibitory Activities of Goyazensolide and Centratherin Isolated From\n                    <i>Eremanthus crotonoides<\/i>\n                    by High\u2010Speed Countercurrent Chromatography"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0049-1132","authenticated-orcid":false,"given":"Natalie Giovanna","family":"da Rocha 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Goytacazes Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6726-273X","authenticated-orcid":false,"given":"Elena B.","family":"Lassunskaia","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia do Reconhecer, Centro de Bioci\u00eancias e Biotecnologia Universidade Estadual do Norte Fluminense Darcy Ribeiro  Campos dos Goytacazes Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6062-4788","authenticated-orcid":false,"given":"Patr\u00edcia","family":"de Homobono de Brito Moura","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Produtos Naturais e Ensaios Biol\u00f3gicos, Departamento de Produtos Naturais e Alimentos Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6917-9323","authenticated-orcid":false,"given":"Ivana Ramos 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