{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T12:46:33Z","timestamp":1775997993443,"version":"3.50.1"},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:00Z","timestamp":1774483200000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Piper cubeba (P. cubeba)<\/jats:italic>\n                    , renowned for its antioxidant properties, has traditionally been used to counteract pro\u2010oxidant effects caused by toxicants. This study investigated the potential immunomodulatory and antioxidant activities of \u03b3\u2010irradiated\n                    <jats:italic>P. cubeba<\/jats:italic>\n                    in a model of rheumatoid arthritis (RA) induced by complete Freund's adjuvant (CFA) in female Wistar rats. RA was induced by a single subcutaneous injection of 0.1 mL of CFA into the right hind footpad. For 3 weeks, arthritic rats received an oral dose of methanolic extract of \u03b3\u2010irradiated\n                    <jats:italic>P. cubeba<\/jats:italic>\n                    (50 mg\/kg body weight\/day). Blood and tissue samples were measured for MMP\u20101, MMP\u20103, ACCP, TNF\u2010\u03b1, IL\u201017, IL\u201010, and NQO1 levels using ELISA, as well as for the oxidative stress marker MDA and the antioxidant enzyme SOD. CFA\u2010induced RA resulted in marked increases in the expression of cyclooxygenase\u20102 (COX\u20102), tumor necrosis factor\u2010alpha, oxidative stress markers, and matrix metalloproteinases. Meanwhile, treatment with irradiated\n                    <jats:italic>P. cubeba<\/jats:italic>\n                    reduced the severity of these changes by increasing anti\u2010inflammatory cytokine IL\u201010 and enhancing FOXP3 expression, a key regulator of T regulatory (Treg) cells, in arthritic rats. These findings demonstrate that irradiated\n                    <jats:italic>P. cubeba<\/jats:italic>\n                    exerts immunomodulatory, antioxidant, and anti\u2010inflammatory effects.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202503288","type":"journal-article","created":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T07:01:53Z","timestamp":1774508513000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Immunomodulatory and Antioxidant Potencies of \u03b3\u2010irradiated Piper cubeba in Complete Freund's Adjuvant\u2010Induced Arthritis in Rats"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7238-3854","authenticated-orcid":false,"given":"Gehan Roushdy","family":"Abdel\u2010Hamid","sequence":"first","affiliation":[{"name":"Radiation Biology Research Department National Centre for Radiation Research and Technology Egyptian Atomic Energy Authority  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Safaa A. E.","family":"Abd El Wahab","sequence":"additional","affiliation":[{"name":"Food Irradiation Research Department National Centre for Radiation Research and Technology Egyptian Atomic Energy Authority  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eman M.","family":"Elgazzar","sequence":"additional","affiliation":[{"name":"Health Radiation Research Department National Centre for Radiation Research and Technology Egyptian Atomic Energy Authority  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayat L.","family":"Soude","sequence":"additional","affiliation":[{"name":"Food Irradiation Research Department National Centre for Radiation Research and Technology Egyptian Atomic Energy Authority  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lobna M.","family":"Anees","sequence":"additional","affiliation":[{"name":"Health Radiation Research Department National Centre for Radiation Research and Technology Egyptian Atomic Energy Authority  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,26]]},"reference":[{"key":"e_1_2_16_2_1","unstructured":"S.Senthelal J.Li S.Ardeshirzadeh andM. 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