{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T16:44:20Z","timestamp":1787503460309,"version":"build-2736575974"},"reference-count":56,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Medicinal plants remain vital to drug discovery due to their diverse secondary metabolites.\n                    <jats:italic>Anonidium mannii<\/jats:italic>\n                    , an underutilized plant traditionally used for fever, diabetes, cancers, and infections, was evaluated for its pharmacological and metabolomic profiles using hexane, chloroform, and butanol\u2010water extracts. Antibacterial activity against six pathogenic bacteria, antioxidant potential (2,2\u2010diphenyl\u20101\u2010picrylhydrazyl (DPPH) assay), and cytotoxicity on Vero and HepG2 cells (MTT (3\u2010[4,5\u2010dimethylthiazol\u20102\u2010yl]\u20102,5 diphenyl tetrazolium bromide) assay) were assessed. Chemical profiling employed integrated LC\u2010MS and GC\u2010MS. The butanol\u2013water extract exhibited strong antibacterial effects (MIC: 0.62\u20135.0 mg\/mL) and potent antioxidant activity (IC\n                    <jats:sub>50<\/jats:sub>\n                    : 0.44 \u00b1 0.05 \u00b5g\/mL), while the hexane extract showed notable selectivity toward HepG2 cells (SI: 1.99). LC\u2010MS revealed bioactive metabolites, including flavonoids and caffeoylquinic acids, most reported here for the first time in\n                    <jats:italic>A. mannii<\/jats:italic>\n                    . GC\u2010MS tentatively identified several bioactive hydrocarbons and esters with known anticancer properties. These results provide evidence of antibacterial, antioxidant, and cytotoxic activities in\n                    <jats:italic>A. mannii<\/jats:italic>\n                    leaf extracts, consistent with its traditional uses.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202503292","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T12:44:29Z","timestamp":1772973869000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Linking Metabolomic Diversity to Pharmacological Potency in\n                    <i>Anonidium mannii<\/i>\n                    Leaf Extracts"],"prefix":"10.1002","volume":"23","author":[{"given":"Olusesan","family":"Ojo","sequence":"first","affiliation":[{"name":"Department of Agriculture and Animal Health University of South Africa  Johannesburg South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garland K.","family":"More","sequence":"additional","affiliation":[{"name":"College of Agriculture and Environmental Sciences Laboratories University of South Africa  Johannesburg South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Prinsloo","sequence":"additional","affiliation":[{"name":"Department of Agriculture and Animal Health University of South Africa  Johannesburg South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants1007135"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1515\/psr\u20102020\u20100207"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0261935"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.2147\/JEP.S379805"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906\u2010023\u201003862\u20108"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2022.e10477"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants13040523"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaim.2022.100676"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.111381"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.596855"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/ddc3010011"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2022.01.010"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11684\u2010021\u20100898\u20106"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2014.10.091"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13020\u2010022\u201000601\u2010y"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202500234"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fochms.2021.100012"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.6165\/tai.2022.67.576"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2020.1758094"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906\u2010024\u201004646\u20104"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.36348\/sjmps.2022.v08i11.008"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants12203629"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.abr.2022.08.007"},{"key":"e_1_2_9_25_1","first-page":"12","article-title":"In\u2010Vitro Antifungal Activity of Methanolic Fractions of Two Cameroonian Medicinal Plants: Anonidium mannii and Xylopia africana (Annonaceae)","volume":"9","author":"Hubert K. 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