{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:26:19Z","timestamp":1791915979921,"version":"4.3.4"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study aims to investigate the therapeutic effect of ethanol extract from fermented broth of\n                    <jats:italic>Acanthopanacis senticosi<\/jats:italic>\n                    Radix et Rhizoma seu Caulis\u2014\n                    <jats:italic>Ganoderma applanatum<\/jats:italic>\n                    (EEA\u2010GF) on IM and clarify its active material basis and mechanism of action. Insomnia (IM) model mice were established by induction with p\u2010chlorophenylalanine (PCPA). The IM status was evaluated through a pentobarbital sodium\u2010induced righting reflex test, hematoxylin\u2010eosin (H&amp;E) staining, and biochemical index detection. The composition of EEA\u2010GF and its blood\u2010migrating components were analyzed based on ultra\u2010performance liquid chromatography\u2010tandem mass spectrometry (UPLC\u2010MS\/MS) technology. Core active components were screened by combining weighted gene co\u2010expression network analysis (WGCNA), network pharmacology, and molecular docking technology. Animal experiments have shown that EEA\u2010GF exhibits excellent therapeutic efficacy in the treatment of IM. A total of 19 compounds were identified from the serum of mice administered with EEA\u2010GF via gavage using UPLC\u2010MS\/MS technology. Through WGCNA, HIF1A, REN, NFE2L2, and PARP1 were determined as core targets. Gene Ontology (GO) enrichment analysis yielded 87 results. In addition to cancer\u2010related pathways, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis mainly included pathways such as the thyroid hormone signaling pathway and neuroactive ligand\u2010receptor interaction. Molecular docking results showed that five components including kaempferol exhibited good binding activity with the aforementioned core targets, indicating that they may be the key material basis for EEA\u2010GF to exert its anti\u2010IM effect. This study is the first to confirm the therapeutic effect of EEA\u2010GF on IM. This finding and the research on its related mechanisms provide a new theoretical basis for the clinical treatment and management of IM, as well as a candidate target with great development prospects.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202503388","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T09:30:38Z","timestamp":1777368638000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Exploring the Mechanism of Sedative and Hypnotic Effects of Fermented Extract From Acanthopanacis senticosi Radix Et Rhizoma Seu Caulis\u2010\n                    <i>Ganoderma applanatum<\/i>\n                    (Pers.) Pat. Through WGCNA Analysis Combined With Network Pharmacology"],"prefix":"10.1002","volume":"23","author":[{"given":"Ying\u2010Ying","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy School of Pharmacy Jiamusi University  Jiamusi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao\u2010Dong","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy School of Pharmacy Jiamusi University  Jiamusi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song\u2010Lin","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy School of Pharmacy Jiamusi University  Jiamusi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin\u2010De","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy School of Pharmacy Jiamusi University  Jiamusi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si\u2010Yu","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy School of Pharmacy Jiamusi University  Jiamusi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9915-8868","authenticated-orcid":false,"given":"Li\u2010Hong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy School of Pharmacy Jiamusi University  Jiamusi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010Cui","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmacy The First Affiliated Hospital of Jiamusi University  Jiamusi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,28]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smrv.2025.102121"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2011.03.013"},{"key":"e_1_2_12_4_1","first-page":"215","volume-title":"Pharmacopoeia of the People's Republic of China","author":"National Pharmacopoeia Committee","year":"2025"},{"key":"e_1_2_12_5_1","first-page":"12","article-title":"Screening of Sedative\u2010Hypnotic Components From Acanthopanax senticosus and Ganoderma applanatum and Preparation Technology of Yogurt","volume":"02","author":"Li C. 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