{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T09:09:15Z","timestamp":1788858555268,"version":"build-2803163510"},"reference-count":72,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Anthyllis henoniana<\/jats:italic>\n                    leaves and flowers were subjected to fractionated extraction using cyclohexane (CYHA), dichloromethane (DCM), ethyl acetate (EtOAc) and methanol (MeOH). The leaves\u2010MeOH extract showed the highest yield and total polyphenol contents (TPC) compared to the flowers. The strongest antiradical activity (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 7.7\u00a0\u00b5g\/mL) was recorded for the leaves compared to flowers (&gt;50\u00a0\u00b5g\/mL). The HPLC\u2010DAD analysis showed the presence of eight and twelve bioactive compounds in the leaf and flower extracts, respectively. The flowers\u2010CYHA extract exhibited the best anti\u20105\u2010lipoxygenase (anti\u20105\u2010LOX) activity (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 15\u00a0\u00b5g\/mL) compared to the leaves (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 42 to 47\u00a0\u00b5g\/mL).\n                    <jats:italic>Anthyllis henoniana<\/jats:italic>\n                    leaves showed greater anti\u2010acetylcholinesterase (anti\u2010AChE) activity than the flowers. The leaves\u2010DCM fraction induced 60 and 70% cell growth inhibition against MCF\u20107 and HCT\u2010116, respectively. Furthermore, the molecular docking investigations supported the in vitro antioxidant and anti\u20105\u2010LOX patterns, revealing that the identified compounds exhibited low binding energies and interacted effectively within the novel human peroxidase (PDB:1PRX) and the crystal structure of soybean lipoxygenase\u2010B (PDB: 2IUJ) enzymes, respectively. The obtained results indicated that the leaves\u2010MeOH extract was the most enriched in polar compounds with high antioxidant and cytotoxic capacities, whereas the flowers\u2010CYHA extracts accumulated nonpolar compounds of an effective anti\u2010inflammatory capacity.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202503501","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T19:15:46Z","timestamp":1786043746000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phytochemical Profiling and Biological Evaluation of\n                    <i>Anthyllis henoniana<\/i>\n                    Organs: Integrated In Vitro and Molecular Docking Insights Into Antioxidant, Anti\u2010Inflammatory, and Cytotoxic Potential"],"prefix":"10.1002","volume":"23","author":[{"given":"Sabrine","family":"Jaber","sequence":"first","affiliation":[{"name":"Laboratoire De Recherche \u201cBiodiversit\u00e9, Mol\u00e9cules et Applications LR22ES02'', Institut sup\u00e9rieur De Biologie Appliqu\u00e9e Universit\u00e9 De Gab\u00e9s Gab\u00e9s Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7686-7597","authenticated-orcid":false,"given":"Rami","family":"Rahmani","sequence":"additional","affiliation":[{"name":"Laboratoire De Recherche \u201cBiodiversit\u00e9, Mol\u00e9cules et Applications LR22ES02'', Institut sup\u00e9rieur De Biologie Appliqu\u00e9e Universit\u00e9 De Gab\u00e9s Gab\u00e9s Tunisia"},{"name":"Become: Technology, Science, AI &amp; Automation Lab  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2439-5145","authenticated-orcid":false,"given":"Jalloul","family":"Bouajila","sequence":"additional","affiliation":[{"name":"Laboratoire De G\u00e9nie Chimique Universit\u00e9 De Toulouse, CNRS, INPT  Toulouse UT France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6473-6143","authenticated-orcid":false,"given":"Mohamed","family":"Debouba","sequence":"additional","affiliation":[{"name":"Laboratoire De Recherche \u201cBiodiversit\u00e9, Mol\u00e9cules et Applications LR22ES02'', Institut sup\u00e9rieur De Biologie Appliqu\u00e9e Universit\u00e9 De Gab\u00e9s Gab\u00e9s Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,6]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"500","volume-title":"Catalogue synonymique comment\u00e9 de la flore de Tunisie","author":"Le Floc'H E.","year":"2011"},{"issue":"2","key":"e_1_2_9_3_1","first-page":"54","article-title":"Dynamic of plant communities in Saharan rangelands of Tunisia","volume":"18","author":"Gamoun M.","year":"2012","journal-title":"Arid Ecosystems"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pld.2018.06.004"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2011.11.010"},{"key":"e_1_2_9_6_1","first-page":"204","volume-title":"Flore succincte des principales plantes pastorales des zones arides et semi\u2010arides de la Tunisie","author":"Chaieb M.","year":"1998"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants10102031"},{"issue":"1","key":"e_1_2_9_8_1","first-page":"49","article-title":"Impact of Acacia tortilis subsp. raddiana on the genetic diversity of its rhizobial symbionts in Tunisian arid soils","volume":"28","author":"Fterich A.","year":"2014","journal-title":"Resource Management"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaridenv.2021.104467"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20101963(05)80053\u20109"},{"issue":"13","key":"e_1_2_9_11_1","first-page":"1222","article-title":"A Review on Trends in Production of Secondary Metabolites From Higher Plants by in Vitro Tissue, Organ and Cell Cultures","volume":"3","author":"Karuppusamy S.","year":"2009","journal-title":"ournal of Medicinal Plants Research"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031\u20109422(88)80254\u20101"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751\u20101097.1999.tb01944.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241310915"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202100345"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40415\u2010023\u201000949\u2010x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2018.08.034"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants12030447"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24043266"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/8516302"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28093947"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfda.2013.11.001"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2025.1615338"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.12944\/CRNFSJ.13.2.27"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants13192748"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23071657"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27217495"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0305\u20101978(02)00050\u20109"},{"key":"e_1_2_9_29_1","first-page":"1173","article-title":"Distribution of Secondary Metabolites in Plants of Quetta\u2010Balochistan","volume":"39","author":"Kayani S. A.","year":"2007","journal-title":"Pakistan Journal of Botany"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3NP00037K"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.5530\/jyp.2024.16.34"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031\u20109422(00)00427\u20101"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.2174\/0115734110301556240605111849"},{"key":"e_1_2_9_34_1","first-page":"411","article-title":"Polydatin: A natural compound with multifaceted anticancer properties","volume":"15","author":"Imtiyaz K.","year":"2024","journal-title":"Journal of Traditional and Complementary Medicine"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.3346"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1080\/10286020.2024.2414196"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4MD00524D"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.202200146"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2004.11.083"},{"issue":"2","key":"e_1_2_9_40_1","first-page":"1","article-title":"Review on Chemical Constituents and Biological Activities of Genus Rumex","volume":"5","author":"Boukhary R. M. K.","year":"2023","journal-title":"BAU Journal Health Wellbeing"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27186043"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13321\u2010016\u20100174\u2010y"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10600\u2010005\u20100200\u20101"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2022.e10467"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.26538\/tjnpr\/v6i1.7"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox13070793"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph19020299"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201004\u20102429"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.2217\/14796694.4.4.475"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2014.05.056"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1055\/a-0626-7426"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28237837"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00217\u2010024\u201004529\u20105"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/jhet.70068"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.2174\/0113816128317849241108064144"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3NJ05476D"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp980230o"},{"issue":"11","key":"e_1_2_9_58_1","first-page":"635","article-title":"Drug Likeness, Targets, Molecular Docking and ADMET Studies for some Indolizine Derivatives","volume":"73","author":"Belal A.","year":"2018","journal-title":"International Journal of Pharmaceutical Sciences"},{"key":"e_1_2_9_59_1","first-page":"1","article-title":"A Clickable Glutathione Approach to Quantify and Profile Reversible Protein S\u2010Glutathionylation","volume":"3","author":"Ndombera F. T.","year":"2020","journal-title":"Journal of Bioinformatics Computational Genomics"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1088\/1755\u20101315\/890\/1\/012021"},{"key":"e_1_2_9_61_1","first-page":"33","article-title":"Pyrazoline B\u2010Paclitaxel or Doxorubicin Combination Drugs Show Synergistic Activity against Cancer Cells: In Silico Study","volume":"17","author":"Wiraswati H. L.","year":"2024","journal-title":"Adv Appl Bioinform Chem"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28083470"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.3382"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41207\u2010024\u201000532\u20100"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2013.01.036"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13065\u2010022\u201000822\u20100"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2025.1583365"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11694-020-00671-2"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2014.01.042"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxrep.2015.05.003"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsb0598\u2010400"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2017.04.005"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo13020281"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202503501","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.202503501","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202503501","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T08:29:36Z","timestamp":1788856176000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202503501"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":72,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/cbdv.202503501"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202503501","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2025-10-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e03501"}}