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Although crude extract of these plants was found to exhibit antimalarial potential, we employed bioassay\u2010guided fractionation to isolate and purify the compounds responsible for the effect. For this purpose, in vitro assays using SYBR Green\u2010I\u2010based fluorescence tests and in silico molecular docking studies were employed. The methanolic extracts of\n                    <jats:italic>A. marmelos<\/jats:italic>\n                    leaves and\n                    <jats:italic>S. aromaticum<\/jats:italic>\n                    flower buds, alongside their fractions (hexane, chloroform, ethyl acetate, and methanol), were appraised against\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    3D7 strain. Further, cytotoxicity was assessed in HeLa cell lines by MTT assay. Comprehensive spectroscopic analyses, including NMR and IR, were carried out for bioactive compound structural characterization. On fractionation, purification, and characterization, four compounds were isolated from\n                    <jats:italic>A. marmelos<\/jats:italic>\n                    leaves and\n                    <jats:italic>S. aromaticum<\/jats:italic>\n                    flower buds, and they exhibited significant antimalarial activity. Lupeol, isolated from\n                    <jats:italic>A. marmelos<\/jats:italic>\n                    , exhibited the most significant effect with 94.0% inhibition and an IC\n                    <jats:sub>50<\/jats:sub>\n                    of 8.73\u00a0\u00b5g\/mL. \u03b2\u2010Sitosterol and 1,3\u2010dimethylpyrimidine\u20102,4(1H,3H)\u2010dione, also isolated from\n                    <jats:italic>A. marmelos<\/jats:italic>\n                    , showed moderate activity. Eugenol, isolated from\n                    <jats:italic>S. aromaticum<\/jats:italic>\n                    , displayed moderate antimalarial activity with an IC\n                    <jats:sub>50<\/jats:sub>\n                    of 19.30\u00a0\u00b5g\/mL. The cytotoxicity investigation indicated a TC\n                    <jats:sub>50<\/jats:sub>\n                    value over 100 \u00b5g\/mL for lupeol, whereas other drugs exhibited mild toxicity. Docking studies revealed binding affinities of \u22129.5 and \u22129.4\u00a0kcal\/mol for lupeol and \u03b2\u2010sitosterol, respectively, toward ribosome inhibitor protein, current candidate drug target of\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    3D7. Lead compounds were also advantageous with drug\u2010likeness and safety profiles. These results scientifically substantiate the conventional utilization of\n                    <jats:italic>A. marmelos<\/jats:italic>\n                    and\n                    <jats:italic>S. aromaticum<\/jats:italic>\n                    as potential sources of antimalarial compounds.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202503548","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T11:00:09Z","timestamp":1772967609000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antimalarial Bioactive Compounds From\n                    <i>Aegle marmelos<\/i>\n                    and\n                    <i>Syzygium aromaticum<\/i>\n                    : In Vitro Evaluation and In Silico Molecular Insights"],"prefix":"10.1002","volume":"23","author":[{"given":"Selvam","family":"Naveenkumar","sequence":"first","affiliation":[{"name":"Department of Biotechnology Faculty of Science and Humanities SRM Institute of Science and Technology (SRMIST) Kattankulathur Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chinnaperumal","family":"Kamaraj","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Faculty of Science and Humanities SRM Institute of Science and Technology (SRMIST) Kattankulathur Chennai Tamil Nadu India"},{"name":"Directorate of Research Interdisciplinary Institute of Indian System of Medicine SRM Institute of Science and Technology Kattankulathur Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pradisha","family":"Prem","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Faculty of Science and Humanities SRM Institute of Science and Technology (SRMIST) Kattankulathur Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajagopalan Vijayalakshmi","family":"Boomija","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Faculty of Science and Humanities SRM Institute of Science and Technology (SRMIST) Kattankulathur Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vetriselvan","family":"Subramaniyan","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences School of Medical and Life Sciences Sunway University  Bandar Sunway Selangor Darul Ehsan Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajappan Chandra Satish","family":"Kumar","sequence":"additional","affiliation":[{"name":"Directorate of Research Interdisciplinary Institute of Indian System of Medicine SRM Institute of Science and Technology Kattankulathur Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chidambaram","family":"Jayaseelan","sequence":"additional","affiliation":[{"name":"Centre For Laboratory Animal Technology and Research Sathyabama Institute of Science and Technology Chennai Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,4]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"WHO World Malaria Report 2020: 20 Years of Global Progress and Challenges.World malaria report 2020: 20 years of global progress and challenges 2020. p.299(Issue December)."},{"key":"e_1_2_10_3_1","unstructured":"WHO World Malaria Report 2023 \u2013 Spread View 2023 https:\/\/www.who.int\/teams\/global\u2010malaria\u2010programme\/reports\/world\u2010malaria\u2010report\u20102023."},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666\u20105247(24)00016\u20108"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936\u2010025\u201005477\u20105"},{"key":"e_1_2_10_6_1","article-title":"Evolutionary Biology of Antimalarial Drug Resistance: Understanding of the Evolutionary Dynamics","volume":"104","author":"Igwe M. 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