{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T16:59:42Z","timestamp":1785085182641,"version":"3.55.0"},"reference-count":62,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:00:00Z","timestamp":1777939200000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Trigonella foenum\u2010graecum<\/jats:italic>\n                    L. seed polysaccharides (FSP) belong to a very important species of biological macromolecules with prebiotic activity, which are the basic substances that have broad application potential in food and pharmaceutical fields. However, systematic studies focusing on the compound enzyme\u2010assisted extraction of FSP and their hepatoprotective effects remain limited. This study aimed to optimize the compound enzyme\u2010assisted extraction of FSP, analyze the structural features of deproteinized FSP (DFSP), and evaluate its therapeutic potential against metabolism\u2010associated fatty liver disease (MAFLD). The results showed that the optimal extraction conditions were an enzymatic hydrolysis temperature of 50\u00b0C, an enzymatic hydrolysis pH of 5.3, an enzymatic hydrolysis time of 1.9\u00a0h, and a liquid\u2010to\u2010solid ratio of 23:1\u00a0mL g\n                    <jats:sup>\u22121<\/jats:sup>\n                    , under which the polysaccharide yield reached 24.88% \u00b1 0.15%. The molecular weight distribution of fenugreek seed polysaccharides was 7157.46, 2785.65, and 642.61\u00a0Da, and they were mainly composed of mannose and galactose. In vivo experiments indicated that DFSP exerted a liver\u2010protective effect on MAFLD mice, accompanied by improvements in lipid metabolic parameters, antioxidant capacity, and inflammatory responses. Therefore, this study provides a scientific basis for further investigations into the fine structure and potential hepatoprotective effects of DFSP.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202503580","type":"journal-article","created":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T11:45:51Z","timestamp":1777981551000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Compound Enzyme\u2010Assisted Extraction and Characterization of\n                    <i>Trigonella foenum\u2010graecum<\/i>\n                    L. Polysaccharides and Their Ameliorative Effects on MAFLD"],"prefix":"10.1002","volume":"23","author":[{"given":"Wen\u2010Xuan","family":"Zhao","sequence":"first","affiliation":[{"name":"Qinghai Provincial Key Laboratory for Tibet Plateau Phytochemistry College of Pharmacy Qinghai Minzu University  Xining China"},{"name":"Qinghai Provincial Pharmacy Experimental Teaching Demonstration Center Qinghai Minzu University Xining China"},{"name":"Key Laboratory of Tibetan Medicine Resources Conservation and Development on the Qinghai\u2010Tibet Plateau (Qinghai Minzu University), State Ethnic Affairs Commission Xining China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Qinghai Provincial Key Laboratory for Tibet Plateau Phytochemistry College of Pharmacy Qinghai Minzu University  Xining China"},{"name":"Qinghai Provincial Pharmacy Experimental Teaching Demonstration Center Qinghai Minzu University Xining 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