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The predictive toxicological effects of\n                    <jats:italic>Cedrela fissilis<\/jats:italic>\n                    were evaluated, and LC\u2013MS\/MS\u2010based chemical profiling was performed. The hydroethanolic extract (HeECf) and ethyl acetate (EA) fraction were assessed in multimodel systems with different levels of biological organization. In\n                    <jats:italic>Allium cepa<\/jats:italic>\n                    , HeECf reduced root growth (29%\u201336%) and the mitotic index (50%\u201374%) at 500\u20131000 \u00b5g\u00b7mL\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    , whereas the EA fraction reduced only the mitotic index (30%\u201362%), with less pronounced effect. Chromosomal alterations were more frequent in HeECf than in EA. In Chinese hamster ovary cells, HeECf reduced cell viability (81%\u201368%) at 800\u20131000 \u00b5g\u00b7mL\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    and compromised membrane integrity, whereas the EA fraction showed IC\n                    <jats:sub>50<\/jats:sub>\n                    &gt; 800 \u00b5g\u00b7mL\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    . In\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    , HeECf completely inhibited pupal development at 1000 \u00b5g\u00b7mL\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    , whereas at 600, 700, and 800 \u00b5g\u00b7mL\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    no toxic effects were observed, also partly related to xenobiotic metabolism. Thus, HeECf exhibited cytotoxic and genotoxic effects that impaired pupal development, likely driven by pentacyclic triterpenoids. In contrast, the EA fraction, enriched in flavonoids, displayed lower toxicity than HeECf. Overall, the toxicity of\n                    <jats:italic>C. fissilis<\/jats:italic>\n                    appears to be mechanistically linked to triterpenoid\u2010mediated cytotoxic and genotoxic damage, contributing to developmental disruption.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202503712","type":"journal-article","created":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T17:19:36Z","timestamp":1779383976000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Cedrela fissilis<\/i>\n                    Vell. Inner Bark: LC\u2013MS\/MS\u2010Based Chemical Profiling and Toxicological Effects"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9831-6664","authenticated-orcid":false,"given":"Thain\u00e1 Pereira","family":"da Silva Cabral","sequence":"first","affiliation":[{"name":"Institute of Chemistry Federal University of Mato Grosso  Cuiab\u00e1 Mato Grosso Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6292-5530","authenticated-orcid":false,"given":"Emanuel Victor","family":"dos Santos Nunes","sequence":"additional","affiliation":[{"name":"Nucleous of Bioassay Biosynthesis and Ecophysiology of Natural Products Institute of Chemistry S\u00e3o Paulo State University  Araraquara S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviani de Brito Massad","family":"Gomes da Silva","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Federal University of Mato Grosso  Cuiab\u00e1 Mato Grosso Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fhelipe Jolner Souza","family":"de Almeida","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Federal University of Mato Grosso  Cuiab\u00e1 Mato Grosso Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2114-1748","authenticated-orcid":false,"given":"Douglas Lisboa","family":"Ramalho","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Federal University of Mato Grosso  Cuiab\u00e1 Mato Grosso Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4564-4614","authenticated-orcid":false,"given":"Jadyellen Rondon","family":"Silva","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Federal University of Mato Grosso  Cuiab\u00e1 Mato Grosso Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3067-380X","authenticated-orcid":false,"given":"Anderson","family":"de Oliveira Souza","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Federal University of Mato Grosso  Cuiab\u00e1 Mato Grosso Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3286-4462","authenticated-orcid":false,"given":"Maria Cristina Fuzari","family":"Bezerra","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Health Sciences Faculty of Medicine Federal University of Mato Grosso  Cuiab\u00e1 Mato Grosso Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2353-0181","authenticated-orcid":false,"given":"Ian","family":"Castro\u2010Gamboa","sequence":"additional","affiliation":[{"name":"Nucleous of Bioassay Biosynthesis and Ecophysiology of Natural Products Institute of Chemistry S\u00e3o Paulo State University  Araraquara S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9341-4605","authenticated-orcid":false,"given":"Helder Lopes","family":"Teles","sequence":"additional","affiliation":[{"name":"Institute of Exact and Natural Sciences Federal University of Rondon\u00f3polis  Rondon\u00f3polis Mato Grosso Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8536-971X","authenticated-orcid":false,"given":"Mayara Peron","family":"Pereira","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Federal University of Mato Grosso  Cuiab\u00e1 Mato Grosso Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,21]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccmp.2024.100129"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfaf004"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.5902\/2179460X13234"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0103\u201050532006000300017"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20100028\u20101088323"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2014.09.035"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.13040\/IJPSR.0975\u20108232.14(2).1016\u201026"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573\u2010022\u201000633\u2010x"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2023.503654"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/286216"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/ftox.2025.1636431"},{"key":"e_1_2_11_13_1","volume-title":"Plantas Medicinais: Arte e Ci\u00eancia","author":"Ferri P. 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